CELLULASE ENZYME AND CITRIC ACID IN PRODUCING IODINE-ENRICHED AGAR POWDER FROM GRACILARIA TENUISTIPITATA
Рубрики: RESEARCH ARTICLE
Аннотация и ключевые слова
Аннотация:
Seaweed is a valuable source of iodine, an essential micronutrient critical for thyroid function. However, conventional iodine fortification methods often result in nutrient loss and environmental issues, necessitating more sustainable alternatives. This study established an environmentally sustainable process to produce iodine-enriched agar powder from Gracilaria tenuistipitata C.F. Chang & B.-M. Xia, 1976, a red seaweed commonly cultivated in Vietnam. The production involved pretreatment, agar extraction, blending of functional ingredients, and organic iodine enrichment. Response Surface Methodology optimized critical factors, including enzyme concentration, citric acid usage, and iodine supplementation. The developed agar-based powder had a stable iodine content (7.42 mg/100 g), protein level (8.51%), and a firm, elastic gel texture. Utilizing MPB cellulase enzyme and citric acid improved the agar yield by ~ 20%, minimized the environmental impact, preserved bioactive compounds, and stabilized iodine. Optimal extraction conditions included cellulase (0.4–0.6%), temperature (45–50°C), and duration (36–48 h). The final optimal formulation combined agar, carrageenan, and gelatin (60:30:10). It was supplemented with 0.2% of sea grapes and 2.5% fermented red algae to significantly enhance iodine retention and sensory quality. Analytical tests (HPLC, UV-Vis) and sensory evaluation verified the product’s quality. The agar powder demonstrated excellent iodine stability and maintained sensory acceptability after six months of refrigeration storage. Integrating cellulase enzymatic treatment, citric acid refinement, and organic iodine enrichment effectively enhanced the nutritional value and addresses iodine deficiency in health-oriented functional foods.

Ключевые слова:
Seaweed, agar powder, iodine, Gracilaria tenuistipitata, processing technology, enrichment, enzymatic treatment
Список литературы

1. Zimmermann MB. Iodine deficiency. Endocrine Reviews. 2009;30(4):376–408. https://doi.org/10.1210/er.2009-0011

2. Otico E. An opportunity for progress: Iodine status, salt iodization and regional disparities in Vietnam. 2025;(Summer):25–33.

3. Darnton-Hill I. Prevalence, causes, and consequences of micronutrient deficiencies. The gap between need and action. In: Mannar MGV, Hurrell RF, editors. Food Fortification in a Globalized World. Hoboken, NJ: Academic Press; 2018, pp. 13–28. https://doi.org/10.1016/B978-0-12-802861-2.00002-X

4. Braye K, Ferguson J, Davis D, Catling C, Monk A, et al. Effectiveness of intrapartum antibiotic prophylaxis for early-onset group B Streptococcal infection: An integrative review. Women and birth. 2018;31(4):244–253. https://doi.org/10.1016/j.wombi.2017.10.012

5. Black MM, Lozoff B, Trude ACB. Nutrition and child development. In: Haith MM, Benson JB, editors. Encyclopedia of infant and early childhood development. San Diego: Academic Press; 2008, pp. 449–459.

6. Nedić O. Iodine: Physiological importance and food sources. eFood. 2023;4(1):e63. https://doi.org/10.1002/efd2.63

7. Kryzhova Y, Antonuk M, Stabnikov V, Stabnikova O. Stability of selenium and iodine in the functional meat products prepared with seaweeds under different cooking procedures. Ukrainian Food Journal. 2021;10(1):136–144. https://doi.org/10.24263/2304-974X-2021-10-1-12

8. Smyth PPA. Iodine, seaweed, and the thyroid. European Thyroid Journal. 2021;10(2):101–108. https://doi.org/10.1159/000512971

9. Ali MQ, Azhar MA, Munaim MSA, Ruslan NF, Alsubhi LM, et al. Seaweed organic compounds source of hydrocolloids and sustainable food packaging: Properties, application, and future direction. Discover Food. 2024;4:101. https://doi.org/10.1007/s44187-024-00173-w

10. Mendes M, Fortunato D, Cotas J, Pacheco D, Morais T, et al. Agar content of estuarine seaweed Gracilaria using different cultivation methods. Applied Food Research. 2022;2(2):100209. https://doi.org/10.1016/j.afres.2022.100209

11. Luo X, Wu Y, Wang S, Xiong Z, Du X, et al. Frontier review of key reduction technologies and resource utilization of waste during the seaweed gel production process. Blue Biotechnology. 2024;1:12. https://doi.org/10.1186/s44315-024-00013-7

12. Milinovic J, Rodrigues C, Diniz M, Noronha JP. Determination of total iodine content in edible seaweeds: Application of inductively coupled plasma-atomic emission spectroscopy. Algal Research. 2021;53:102149. https://doi.org/10.1016/j.algal.2020.102149

13. Mantri VA, Gajaria TK, Rathod SG, Prasad K. A mini review on iodinophyte seaweed resources of India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2024;95:463–474. https://doi.org/10.1007/s40011-024-01571-x

14. Retiaty F, Nurjanah N. Iodine content and acceptability of seaweed flour from various types of seaweed, drying methods, and types of fishy odor removal. IOP Conference Series: Earth and Environmental Science. 2023;1273:012021. https://doi.org/10.1088/1755-1315/1273/1/012021

15. Correia H, Soares C, Morais S, Pinto E, Marques A, et al. Seaweeds rehydration and boiling: Impact on iodine, sodium, potassium, selenium, and total arsenic contents and health benefits for consumption. Food and Chemical Toxicology. 2021;155:112385. https://doi.org/10.1016/j.fct.2021.112385

16. Qin Y. Seaweed hydrocolloids as thickening, gelling, and emulsifying agents in functional food products. Bioactive seaweeds for food applications. Amsterdam: Elsevier; 2018, pp. 135–152.

17. Qin Y, Lu X, Sun N, Rogers RD. Dissolution or extraction of crustacean shells using ionic liquids to obtain high molecular weight purified chitin and direct production of chitin films and fibers. Green Chemistry. 2010;12:968–971. https://doi.org/10.1039/C003583A

18. Kiruthika M, Shivaswamy MS. Extraction of provitamin and non-provitamin carotenoid using conventional and modern extraction methods – A review. Food and Humanity. 2024;2:100241. https://doi.org/10.1016/j.foohum.2024.100241

19. Mir SA, Rizwan D, Bakshi RA, Wani SM, Masoodi FA. Extraction of carotenoids from agro-industrial waste. Extraction of Natural Products from Agro-Industrial Wastes. Amsterdam: Elsevier; 2023, pp. 157–178.

20. Mohibbullah M, Talha MA, Baten MA, Wares NA, Choi JS. Yield optimization, physicochemical characterizations, and antioxidant properties of food grade agar from Gracilaria tenuistipitata of Cox’s Bazar coast, Bangladesh. Food Science & Nutrition. 2023;11(6):2852–2863. https://doi.org/10.1002/fsn3.3265

21. Rhein-Knudsen N, Ale MT, Meyer AS. Seaweed hydrocolloid production: An update on enzyme assisted extraction and modification technologies. Marine Drugs. 2015;13(6):3340–3359. https://doi.org/10.3390/md13063340

22. Trigueros E, Sanz MT, Filipigh A, Beltrán S, Riaño P. Enzymatic hydrolysis of the industrial solid residue of red seaweed after agar extraction: Extracts characterization and modelling. Food and Bioproducts Processing. 2021;126:356–366. https://doi.org/10.1016/j.fbp.2021.01.014

23. Li Y, Zhao M, Gomez LP, Senthamaraikannan R, Padamati RB, et al. Investigation of enzyme-assisted methods combined with ultrasonication under a controlled alkali pretreatment for agar extraction from Gelidium sesquipedale. Food Hydrocolloids. 2021;120:106905. https://doi.org/10.1016/j.foodhyd.2021.106905

24. Belattmania Z, Bhaby S, Nadri A, Khaya K, Bentiss F, et al. Gracilaria gracilis (Gracilariales, Rhodophyta) from Dakhla (Southern Moroccan Atlantic Coast) as source of agar: Content, chemical characteristics, and gelling properties. Marine Drugs. 2021;19(12):672. https://doi.org/10.3390/md19120672

25. Shukla P, Bajwa U, Sharma S. Optimization of the level of ingredients for functional dairy beverage using response surface methodology (RSM). Acta Alimentaria An International Journal of Food Science. 2014;43:402–411. https://doi.org/10.1556/aalim.43.2014.3.6

26. Carpena M, Caleja C, Pereira E, Pereira C, Ćirić A, et al. Red seaweeds as a source of nutrients and bioactive compounds: Optimization of the extraction. Chemosensors. 2021;9(6):132. https://doi.org/10.3390/chemosensors9060132

27. Neves C, Morais S, Vale T, Soares C, Grosso C, et al. Exploring the bioactive potential of Gracilaria gracilis: An extraction optimization study using response surface methodology. Biology and Life Sciences Forum. 2023;26(1):13. https://doi.org/10.3390/Foods2023-15026

28. Chen Z, Zhang L, Liu Y, Wen Y, Shan S, et al. Seaweed as a sustainable future food source. International Journal of Food Science and Technology. 2024;59(3):1237–1247. https://doi.org/10.1111/ijfs.16910

29. Charoenphun N, Noonim P, Lekjing S, Nitikornwarakul C, Pham NH, et al. Physicochemical properties, antioxidant activity, and flavor profile of strawberry fruit-based novel drinking jelly made with Gracilaria fisheri seaweed as a gelling agent at varying concentrations. Gels. 2025;11(1):54. https://doi.org/10.3390/gels11010054

30. Yeh TS, Hung NH, Lin TC. Analysis of iodine content in seaweed by GC-ECD and estimation of iodine intake. Journal of Food and Drug Analysis. 2014;22(2):189–196. https://doi.org/10.1016/j.jfda.2014.01.014

31. Jayakody MM, Kaushani KG, Vanniarachchy MPG, Wijesekara I. Hydrocolloid and water soluble polymers used in the food industry and their functional properties: A review. Polymer Bulletin. 2023;80:3585–3610. https://doi.org/10.1007/s00289-022-04264-5

32. Aakre I, Tveit IB, Myrmel LS, Fjære E, Ballance S, et al. Bioavailability of iodine from a meal consisting of sushi and a wakame seaweed salad – A randomized crossover trial. Food Science & Nutrition. 2023;11(12):7707–7717. https://doi.org/10.1002/fsn3.3689

33. Li N, Geng Z, Guo Y, Dai X, Zhu W, et al. Evaluation of the bioavailability of iodine and arsenic in raw and cooked Saccharina japonica based on simulated digestion/Caco-2 cell model. Foods. 2024;13(18):2864. https://doi.org/10.3390/foods13182864

34. Ameye L, de Brouwer S, Gilliland DL, Heckmann J, Janakiraman K, et al. Stability of nutrients in complex liquid and powder food matrices: Learnings from shelf-life studies in foods for special medical purposes. Current Research in Food Science. 2025;10:101055. https://doi.org/10.1016/j.crfs.2025.101055

35. Araki Y, Zeng M, Zhang M, Huganir RL. Rapid dispersion of SynGAP from synaptic spines triggers AMPA receptor insertion and spine enlargement during LTP. Neuron. 2015;85(1):173–189. https://doi.org/10.1016/j.neuron.2014.12.023

36. Glicksman MA, Sanes JR. Differentiation of motor nerve terminals formed in the absence of muscle fibres. Journal of Neurocytology. 1983;12:661–671. https://doi.org/10.1007/bf01181529


Войти или Создать
* Забыли пароль?