Aim: to evaluate separately the fractional composition of dust particles (PM – particle matter) micro- (from 10 μm to 100 nm, PM10) and nanofractions (less than 100 nm, PM0.1), as well as their biological effect by the DNA comet method on a culture of lung fibroblasts MRC-5. The most abundant were 1.1-1.3 µm and 50-80 nm particles in micro- and nanofractions, respectively. A high level of DNA disease is found in samples exposed to PM0.1 in searches with PM10.
dust particles, coal-rock particles, PM10, PM0.1, DNA comets, TEM, MRC-5
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