Moscow, Moscow, Russian Federation
Ice cream is a popular cold dessert. Its air phase consists of tiny bubbles with an average diameter of 15–60 µm. New ice cream formulations depend on the way the composition and production factors affect the air phase. As a result, ice cream producers need new time-saving and reliable methods to determine dispersion. The research objective was to create a computer program for marking the position of centers and diameter of air bubbles on microscopic images of a bounding circle type. The review part included 20 years of Russian and English publications on microscopic research methods in ice cream production indexed in Web of Science and Russian Research Citation Index. Microscopic images of ice cream air phase were obtained using an Olympus CX41RF microscope with a magnification of ×100. The automatic markup program employed the Python programming language, the Keras machine learning library, and the TensorFlow framework. The models were trained using the NVIDIA GTX video accelerator. The review showed that the dispersion of ice cream air phase depends on its composition and the freezing parameters whereas bubble formation is usually described in line with the existing foaming theories. A training data set was obtained by manual labeling of microscopic images. The optimal number channels in the convolutional layers of a neural network with LeNet-type architecture was determined, which made it possible to classify images as spheres or non-spheres with an accuracy of ≥ 0.995. The sliding window method helped to determine the limits of the neural network triggering for the sliding window method were determined, which reached 7.5% of the diameter with lateral displacement and 12.5% with scaling. The developed algorithm automatically marked bubbles on microscopic images. The error in determining the average diameter was below 1.8%. The new method for automated calculation of the number and diameter of air bubbles in ice cream proved to be user-friendly. It can be found in public domain, and researchers are free to adapt it to solve various computer vision issues.
Ice cream, microscopy, machine learning, markup, bounding circle, air bubbles
1. Genovese A, Balivo A, Salvati A, Sacchi R. Functional ice cream health benefits and sensory implications. Food Research International. 2022;161. https://doi.org/10.1016/j.foodres.2022.111858
2. Akbari M, Eskandari MH, Davoudi Z. Application and functions of fat replacers in low-fat ice cream: A review. Trends in Food Science and Technology. 2019;86:34-40. https://doi.org/10.1016/j.tifs.2019.02.036
3. Gheisari HR, Heydari S, Basiri S. The effect of date versus sugar on sensory, physicochemical, and antioxidant properties of ice cream. Iranian Journal of Veterinary Research. 2020;21(1):9-14.
4. Arslaner A, Salik MA. Functional ice cream technology. Akademik Gıda. 2020;18(2):180-189. https://doi.org/10.24323/akademik-gida.758835
5. Ademosun AO. Glycemic properties of soursop-based ice cream enriched with moringa leaf powder. Foods and Raw Materials. 2021;9(2):207-214. https://doi.org/10.21603/2308-4057-2021-2-207-214
6. Wu B, Freire DO, Hartel RW. The effect of overrun, fat destabilization, and ice cream mix viscosity on entire meltdown behavior. Journal of Food Science. 2019;84(9):2562-2571. https://doi.org/10.1111/1750-3841.14743
7. Cheng J, Ma Y, Li X, Yan T, Cui J. Effects of milk protein-polysaccharide interactions on the stability of ice cream mix model systems. Food Hydrocolloids. 2015;45:327-336. https://doi.org/10.1016/j.foodhyd.2014.11.027
8. da Silva Faresin L, Devos RJB, Reinehr CO, Colla LM. Development of ice cream with reduction of sugar and fat by the addition of inulin, Spirulina platensis or phycocyanin. International Journal of Gastronomy and Food Science. 2022;27. https://doi.org/10.1016/j.ijgfs.2021.100445
9. Chang Y, Hartel RW. Development of air cells in a batch ice cream freezer. Journal of Food Engineering. 2002;55(1):71-78. https://doi.org/10.1016/S0260-8774(01)00243-6
10. Warren MM, Richard WH. Structural, compositional, and sensorial properties of United States commercial ice cream products. Journal of Food Science. 2014;79(10):E2005-E2013. https://doi.org/10.1111/1750-3841.12592
11. Yan G, Cui Y, Lia D, Ding Y, Han J, Wang S, et al. The characteristics of soybean protein isolate obtained by synergistic modification of high hydrostatic pressure and phospholipids as a promising replacement of milk in ice cream. LWT. 2022;160. https://doi.org/10.1016/j.lwt.2022.113223
12. Shokhalova VN, Kusin AA, Shokhalov VA, Kostyukov EM. Investigation of ice-cream air phase with a curd whey NF concentrate. Molochnokhozayistvenny Vestnik. 2017;26(2):130-137. (In Russ.). https://www.elibrary.ru/ZBFZXF
13. Gurskiy IA, Tvorogova AA, Shobanova TV. The condition of the structure of the thawed aerated sour-milk desserts during its storage. Proceedings of the Voronezh State University of Engineering Technologies. 2020;82(2):94-100. (In Russ.). https://doi.org/10.20914/2310-1202-2020-2-94-100
14. Landikhovskaya AV, Tvorogova AA, Kazakova NV, Gursky IA. The effect of trehalose on dispersion of ice crystals and consistency of low-fat ice cream. Food Processing: Techniques and Technology. 2020;50(3):450-459. (In Russ.). https://doi.org/10.21603/2074-9414-2020-3-450-459
15. Gurskiy IA, Tvorogova AA. The effect of whey protein concentrates on technological and sensory quality indicators of ice cream. Food Processing: Techniques and Technology. 2022;52(3):439-448. (In Russ.). https://doi.org/10.21603/2074-9414-2022-3-2376
16. Jing X, Chen Z, Tang Z, Tao Y, Huang Q, Wu Y, et al. Preparation of camellia oil oleogel and its application in an ice cream system. LWT. 2022;169. https://doi.org/10.1016/j.lwt.2022.113985
17. Schroeder AB, Dobson ETA, Rueden CT, Tomancak P, Jug F, Eliceiri KW. The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis. Protein Science. 2021;30(1):234-249. https://doi.org/10.1002/pro.3993
18. Ilonen J, Juránek R, Eerola T, Lensu L, Dubská M, Zemčík P, et al. Comparison of bubble detectors and size distribution estimators. Pattern Recognition Letters. 2018;101:60-66. https://doi.org/10.1016/j.patrec.2017.11.014
19. Cui Y, Li C, Zhang W, Ning X, Shi X, Gao J, et al. A deep learning-based image processing method for bubble detection, segmentation, and shape reconstruction in high gas holdup sub-millimeter bubbly flows. Chemical Engineering Journal. 2022;449. https://doi.org/10.1016/j.cej.2022.137859
20. Cerqueira RFL, Paladino EE. Development of a deep learning-based image processing technique for bubble pattern recognition and shape reconstruction in dense bubbly flows. Chemical Engineering Science. 2021;230. https://doi.org/10.1016/j.ces.2020.116163
21. Jabarkhyl S, Barigou M, Zhu S, Rayment P, Lloyd DM, Rossetti D. Foams generated from viscous non-Newtonian shear-thinning liquids in a continuous multi rotor-stator device. Innovative Food Science and Emerging Technologies. 2020;59. https://doi.org/10.1016/j.ifset.2019.102231
22. Cook KLK, Hartel RW. Mechanisms of ice crystallisation in ice cream production. Comprehensive Reviews in Food Science and Food Safety. 2010;9(2):213-222. https://doi.org/10.1111/j.1541-4337.2009.00101.x
23. Caillet A, Cogné C, Andrieu J, Laurent P, Rivoire A. Characterization of ice cream structure by direct optical microscopy. Influence of freezing parameters. LWT - Food Science and Technology. 2003;36(8):743-749. https://doi.org/10.1016/S0023-6438(03)00094-X
24. Eisner MD, Wildmoser H, Windhab EJ. Air cell microstructuring in a high viscous ice cream matrix. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2005;263(1-3):390-399. https://doi.org/10.1016/j.colsurfa.2004.12.017
25. Parra ODH, Ndoye F-T, Benkhelifa H, Flick D, Alvarez G. Effect of process parameters on ice crystals and air bubbles size distributions of sorbets in a scraped surface heat exchanger. International Journal of Refrigeration. 2018;92:225-234. https://doi.org/10.1016/j.ijrefrig.2018.02.013
26. Goraya RK, Singla M, Bajwa U, Kaur A, Pathania S. Impact of sodium alginate gelling and ingredient amalgamating order on ingredient interactions and structural stability of ice cream. LWT. 2021;147. https://doi.org/10.1016/j.lwt.2021.111558
27. Akalın AS, Kesenkas H, Dinkci N, Unal G, Ozer E, Kınık O. Enrichment of probiotic ice cream with different dietary fibers: Structural characteristics and culture viability. Journal of Dairy Science. 2018;101(1):37-46. https://doi.org/10.3168/jds.2017-13468
28. Samakradhamrongthai RS, Jannu T, Supawan T, Khawsud A, Aumpa P, Renaldi G. Inulin application on the optimization of reduced-fat ice cream using response surface methodology. Food Hydrocolloids. 2021;119. https://doi.org/10.1016/j.foodhyd.2021.106873
29. Narala VR, Orlovs I, Jugbarde MA, Masin M. Inulin as a fat replacer in pea protein vegan ice cream and its influence on textural properties and sensory attributes. Applied Food Research. 2022;2(1). https://doi.org/10.1016/j.afres.2022.100066
30. Baldominos A, Saez Y, Isasi P. A survey of handwritten character recognition with MNIST and EMNIST. Applied Sciences. 2019;9(15). https://doi.org/10.3390/app9153169
31. Rosebrock A. Deep learning for computer vision with python: Starter bundle. PyImageSearch; 2017. 330 p.
32. Prashanth DS, Mehta RVK, Ramana K, Bhaskar V. Handwritten Devanagari Character Recognition using modified lenet and alexnet convolution neural networks. Wireless Personal Communications. 2022;122:349-378. https://doi.org/10.1007/s11277-021-08903-4