Dairy industry Dairy industry Молочная промышленность 1019-8946 86478 10.21603/1019-8946-2024-4-4 Научная статья Research Article Научная статья Criteria for evaluating the antioxidant properties of secondary plant metabolites in the fortification of dairy products Критерии оценки антиоксидантных свойств вторичных метаболитов при обогащении молочных продуктов https://orcid.org/0000-0002-9546-6633 Ле Виолета Мироновна Le Violetta M. ya808@yandex.ru https://orcid.org/0000-0002-5630-3196 Просеков Александр Юрьевич Prosekov Alexander Yu.

доктор технических наук;

doctor of technical sciences;

 https://orcid.org/0000-0003-3214-8889 Сухих Андрей Сергеевич Sukhikh Andrey S. https://orcid.org/0000-0002-3536-562X Милентьева Ирина Сергеевна Milentyeva Irina S. irazumnikova@mail.ru https://orcid.org/0000-0002-1779-4332 Юстратов Владимир Петрович Yustratov Vladimir P. Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation 15 08 2024 15 08 2024 4 32 40 03 02 2024 30 07 2024 https://moloprom.kemsu.ru/en/nauka/article/86478/view

Функциональные продукты, содержащие природные антиоксиданты, способствуют улучшению показателей здоровья человека и тем самым способствуют увеличению продолжительности жизни. Молоко является ценным солюбилизатором, способствующим растворению труднорастворимых (липофильных) веществ в жидкости. В работе представлены результаты исследования в условиях высокоэффективной жидкостной хроматографии с амперометрическим детектированием антиоксидантных свойсв вторичных метаболитов растений для обогащения молочных продуктов. Объектами исследования являлись биологически активные соединения, выделенные из экстрактов каллусных культур растений, выращенных в условиях in vitro на жидких питательных средах. Применение высокоэффективной жидкостной хроматографии с амперометрическим детектором для анализа антиоксидантных свойств биологически активных веществ растительного происхождения позволило определить из 8 видов индивидуальных соединений с выраженным потенциалом биологической активности следующие вещества: рутин, розмариновая кислота из Pulmonaria officinalis, транс-коричная кислота из Scutellaria baicalensis, рутин из Filipéndula ulmária. Данные соединения способны эффективно ингибировать свободнорадикальные процессы. Применение высокоэффективной жидкостной хроматографии с амперометрическим детектированием может использоваться как критерий оценки антиоксидантных свойств биологически активных веществ, выделенных из экстрактов каллусных культур.

Functional products containing natural antioxidants contribute to the improvement of human health indicators, and thus contribute to an increase in life expectancy. Milk is a valuable solubilizer that promotes the dissolution of insoluble substances in a liquid. The paper presents the results of a study under conditions of high-performance liquid chromatography (HPLC) with amperometric detection of antioxidant properties of secondary plant metabolites for the enrichment of dairy products. The objects of the study were biologically active compounds isolated from extracts of callus cultures of plants grown in vitro on liquid nutrient media. The use of HPLC chromatography with an amperometric glass-carbon detector to analyze the antioxidant properties of biologically active substances of plant origin made it possible to identify the following substances from 8 types of individual compounds with a pronounced potential for biological activity: rutin, rosemary acid from Pulmonaria officinalis, trans-cinnamic acid from Scutellaria baicalensis, rutin from Filipéndula ulmária. These compounds are able to effectively inhibit free radical processes in dairy products. The use of high-performance liquid chromatography with amperometric detection can be used as a criterion for evaluating the antioxidant properties of biologically active substances isolated from extracts of callus cultures.

 молочные продукты каллусные культуры биологически активные вещества антиоксидантная активность высокоэффективная хроматография амперометрическое детектирование dairy products; callus cultures; antioxidant activity; highly effective chromatography; biologically active substances Работа выполнена в рамках государственного задания по теме «Разработка биологически активных добавок, состоящих из метаболитов растительных объектов in vitro, для защиты населения от преждевременного старения» (проект FZSR-2024-0008).

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