Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 84596 10.21603/2074-9414-2024-2-2501 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Screening and Profiling the Antioxidant Properties of Psychrophilic Microalgae and Cyanobacteria from the Baltic Sea Скрининг и характеристика антиоксидантных свойств психрофильных микроводорослей и цианобактерий Балтийского моря https://orcid.org/0000-0003-0603-7456 Долганюк Вячеслав Федорович Dolganyuk Vyacheslav F. https://orcid.org/0000-0001-7910-8388 Сухих Станислав Алексеевич Sukhikh Stanislav A. stas-asp@mail.ru https://orcid.org/0000-0003-0442-5471 Каширских Егор Владимирович Kashirskich Egor V. https://orcid.org/0000-0003-4107-7277 Ульрих Елена Викторовна Ulrikh Elena V. Кремлева Ольга Евгеньевна Kremleva Olga E. https://orcid.org/0000-0002-4921-8997 Бабич Ольга Олеговна Babich Olga O. olich.43@mail.ru Балтийский федеральный университет имени Иммануила Канта Калининград Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Балтийский федеральный университет имени Иммануила Канта Калининград Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Балтийский федеральный университет имени Иммануила Канта Калининград Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Калининградский государственный технический университет Калининград Россия Kaliningrad State Technical University Kaliningrad Russian Federation Гродненский государственный университет имени Янки Купалы Гродно Беларусь Yanka Kupala State U niversity of Grodno Grodno Belarus Балтийский федеральный университет имени Иммануила Канта Калининград Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation 03 07 2024 03 07 2024 54 2 212 221 17 03 2023 05 09 2023 https://fptt.ru/en/issues/22705/22642/

Получение комплекса биологически активных веществ с антиоксидантной активностью из психрофильных микроводорослей и цианобактерий вызывает научный интерес, расширяет область их промышленного применения и открывает новые перспективы их использования. Целью работы являлся сбор и идентификация психрофильных микроводорослей и цианобактерий Балтийского моря, а также изучение их соединений, которые проявляют антиоксидантные свойства. Объекты исследования – образцы психрофильных микроводорослей (Skeletonema pseudocostatum, Thalassiosira pseudonana и Fragilariopsis kerguelensis) и цианобактерий (Aphanizomenon gracile и Anabaena cylindrica). Образцы отобрали из природных источников в акватории Балтийского моря в Калининградской области (Куршский залив и Балтийский залив) в период с марта по май 2022 г. Анализ выделенных чистых культур осуществляли методами Пастера и проточной цитофлуориметрии. Антиоксидантную активность психрофильных микроводорослей и цианобактерий определяли путем анализа активности поглощения радикалов, восстанавливающей способности и хелатной активности с помощью спектрофотометрии. Наибольшей антиоксидантной активностью по трем методам анализа обладала психрофильная микроводоросль S. pseudocostatum, которая по методу ABTS составила 17,62 мкмоль/г эквивалентов тролокса, по методу DPPH 58,16 мкмоль/г эквивалентов тролокса, по методу FRAP 3,91 мкмоль/г эквивалентов тролокса. Антиоксидантная активность T. pseudonana равна 12,08, 12,42 и 3,13 мкмоль/г эквивалентов тролокса. Для психрофильной микроводоросли F. kerguelensis этот показатель составил 13,53, 11,84 и 1,09 мкмоль/г эквивалентов тролокса. Антиоксидантная активность психрофильной цианобактерии A. gracile равна 15,73, 19,89 и 2,47 мкмоль/г эквивалентов тролокса. Значения антиоксидантной активности психрофильной цианобактерии A. cylindrica составили 12,62, 13,16 и 2,16 мкмоль/г эквивалентов тролокса. Отобрали образцы психрофильных микроводорослей и цианобактерий из природных источников в акватории Балтийского моря (Калининградская область), которые продуцируют компоненты с высокой антиоксидантной активностью. Полученные результаты могут стать основой для разработки новой фармацевтической субстанции.

At present, the issue of obtaining a complex of biologically active substances with antioxidant activity from psychrophilic Psychrophilic microalgae and cyanobacteria are a prospective source of biologically active antioxidant substances. New antioxidant complexes could expand the scope of their industrial application. The research objective was to identify psychrophilic microalgae and cyanobacteria from the Baltic Sea in order to study their antioxidant properties. The research featured psychrophilic microalgae and cyanobacteria obtained from the Curonian Lagoon and the Baltic Bay in the Baltic Sea, Kaliningrad Region, Russia, in March – May 2022. The authors used the Pasteur method and the flow cytometry method to isolate pure cultures of psychrophilic microalgae and cyanobacteria. The method of spectrophotometry made it possible to study the antioxidant activity by analyzing radical scavenging, reducing ability, and chelating. The psychrophilic microalga Skeletonema pseudocostatum demonstrated the highest antioxidant activity in all three methods: 17.62 μmol/g trolox equivalents according to the ABTS method, 58.16 μmol/g trolox equivalents according to the DPPH method, and 3.91 μmol/g trolox equivalents according to the FRAP method. The psychrophilic microalga Thalassiosira pseudonana exhibited the following values of antioxidant activity: 12.08, 12.42, and 3.13 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic microalgae Fragilariopsis kerguelensis was 13.53, 11.84, and 1.09 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic cyanobacterium Aphanizomenon gracile was 15.73, 19.89, and 2.47 μmol/g trolox equivalents, respectively. The antioxidant activity of the psychrophilic cyanobacterium Anabaena cylindrica was 12.62, 13.16, and 2.16 μmol/g trolox equivalents, respectively. The samples of psychrophilic microalgae and cyanobacteria obtained from natural environment in the Russian Baltic Sea demonstrated good antioxidant properties, which makes them a potential raw material for new pharmaceutical substances.

 Skeletonema pseudocostatum Thalassiosira pseudonana Fragilariopsis kerguelensis Aphanizomenon gracile Anabaena cylindrica микроводоросли биологически активные вещества скрининг идентификация антиоксидантная активность Skeletonema pseudocostatum Thalassiosira pseudonana Fragilariopsis kerguelensis Aphanizomenon gracile Anabaena cylindrica microalgae biologically active substances screening identi fication antioxidant activity Работа выполнена при финансовой поддержке Министерства науки и высшего образования Российской Федерации (Минобрнауки России) (грант Президента Российской Федерации), проект № МК-484.2022.1.4 (соглашение № 075-15-2022-393). The research was supported by the Presidential Grant from the Ministry of Science and Higher Education of the Russian Federation (Minobrnauki), project no. MK-484.2022.1.4, agreement no. 075-15-2022-393.

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