Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 72962 10.21603/2074-9414-2023-4-2475 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo Биофункциональная активность in vivo хлорогеновой кислоты и биоханина А, выделенных из экстрактов каллусной культуры Trifolium pratense L. https://orcid.org/0000-0002-3536-562X Милентьева Ирина Сергеевна Milentyeva Irina S. irazumnikova@mail.ru https://orcid.org/0000-0002-4552-7418 Веснина Анна Дмитриевна Vesnina Anna D. koledockop1@mail.ru https://orcid.org/0000-0002-8071-4411 Федорова Анастасия Михайловна Fedorova Anastasiya M. https://orcid.org/0000-0002-4704-484X Остапова Елена Владимировна Ostapova Elena V. https://orcid.org/0000-0003-0166-2527 Ларичев Тимофей Альбертович Larichev Timothy A. Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation Кемеровский государственный университет Кемерово Россия Kemerovo State University Kemerovo Russian Federation 30 12 2023 30 12 2023 53 4 754 765 02 05 2023 04 07 2023 https://fptt.ru/en/issues/22269/22242/

Полифенолы представляют интерес как потенциальные нейропротекторы, которые увеличивают продолжительность жизни и замедляют старение. Перспективным источником биологически активных веществ является клевер луговой (Trifolium pratense L.), в экстрактах которого присутствуют биоханин А и хлорогеновая кислота. Цель работы – установить наличие/отсутствие влияния полифенолов, выделенных из экстрактов каллусных культур T. pratense L. на экспрессию генов SOD-3 и HSP-16.2 и антиамилойдной активности с помощью модельных организмов – нематод Caenorhabditis elegans. Объектами исследования являлись хлорогеновая кислота и биоханин А (200, 100, 50 и 10 мкМ) чистотой 95 %, которые выделили из экстрактов каллусов клевера лугового. Влияние полифенолов на экспрессию SOD-3 и HSP-16.2 оценивали при тепловом стрессе (35 °С) в течение 5 и 2 ч соответственно, используя C. elegans N2 Bristol. Нейропротекторную активность оценивали по количеству парализованных C. elegans CL4176 после 18, 40 и 62 ч инкубации. Установлено, что дозазависимый эффект между концентрацией биологически активных веществ и процентом парализованных нематод наблюдался при 18 ч культивирования. Максимальные результаты уменьшения фенотипа парализации наблюдались при добавлении растворов концентрацией 200 мкМ. Активность 200 мкМ биохнина А была в 1,18 раз выше 200 мкМ раствора хлорогеновой кислоты. Растворы биоханина А увеличивали экспрессию SOD-3 только в 3,7 раз в сравнении с контролем. Полученные результаты показали, что исследуемые биологически активные вещества проявляли относительную нейропротекторную активность и способность влиять на экспрессию гена антиоксидантной защиты организма, используя модельный объект C. elegans.

Polyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of polyphenols extracted from red clover callus cultures on SOD-3 and HSP-16.2 genes, as well as their anti-amyloid potential. The chlorogenic acid and biochanin A (200, 100, 50, and 10 µM) with a purity of 95% were isolated from callus extracts of T. pratense L. The effect of polyphenols on SOD-3 and HSP-16.2 was assessed after 5 and 2 h of heat stress (35°C), respectively, using C. elegans N2 Bristol as model organism. The neuroprotective potential was measured by counting paralyzed nematodes after 18, 40, and 62 h of incubation. The research established a dose-dependent effect between the concentration of biologically active substances and the percentage of paralyzed nematodes after 18 h of cultivation. The lowest paralysis phenotype count occurred at a concentration of 200 μM. The activity of 200 μM biochnin A was 1.18 times as high as that of a 200 μM chlorogenic acid solution. Biochanin A solutions increased SOD-3 expression by 3.7 times, compared to the control. The biologically active substances exhibited relative neuroprotective activity and affected the expression of antioxidant defense gene in C. elegans.

 Старение болезнь Альцгеймера нейропротекторы полифенолы хлорогеновая кислота биоханин А Caenorhabditis elegans β-амилоидный пептид Aging Alzheimer's disease neuroprotectors polyphenols chlorogenic acid biochanin A Caenorhabditis elegans β-amyloid peptide Работа выполнена в рамках государственного задания по теме «Полифенолы растений СФО: оценка молекулярной и пространственной структуры веществ, характеристика биофункциональных свойств и токсикологических показателей безопасности на модельных системах in vivo» (проект FZSR-2023-0002) с использованием оборудования ЦКП «Инструментальные методы анализа в области прикладной биотехнологии» на базе Кемеровского государственного университета (КемГУ). The research was part state task FZSR-2023-0002 “Polyphenols from Siberian Federal District plants: assessment of molecular and spatial structure, characterization of biofunctional properties, and toxicological safety indicators using in vivo model systems”. It involved the Shared Use Center for Instrumental Analysis in Applied Biotechnology, Kemerovo State University (KemSU).

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