Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 72954 10.21603/2074-9414-2023-4-2469 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Cultivating Limnospira fusiformis from Lake Solenoye, Omsk Технология культивирования Limnospira fusiformis из озера Соленого (г. Омск) https://orcid.org/0000-0001-7226-5962 Молибога Елена Александровна Moliboga Elena A. ea.moliboga@omgau.org https://orcid.org/0000-0003-2406-4319 Баженова Ольга Прокопьевна Bazhenova Olga P. Омский государственный технический университет Омск Россия Omsk State Technical University Omsk Russian Federation Омский государственный аграрный университет имени П. А. Столыпина Омск Россия P.A. Stolypin Omsk State Agrarian University Omsk Russian Federation 30 12 2023 30 12 2023 53 4 689 697 23 03 2023 02 05 2023 https://fptt.ru/en/issues/22269/22216/

При поиске альтернативных источников продовольствия среди возобновляемых биоресурсов большое внимание уделяется изучению пищевой ценности микроводорослей, в том числе нитчатых цианопрокариот. Фитомасса является источником протеинов и биологически активных веществ и может быть использована в качестве биодобавки, т. к. обладает ценными питательными свойствами. Цель исследования – разработать технологию культивирования штамма Limnospira fusiformis O9.13F, выделенного из воды озера Соленого (г. Омск, Россия), для получения наибольших объемов экологически чистой фитомассы, пригодной для пищевых целей. Объект исследования – нитчатая цианопрокариота L. fusiformis штамма O9.13F. Выделение чистой культуры штамма L. fusiformis O9.13F проводили микропипеточным способом из проб воды, отобранных в озере Соленом после окончания цветения воды. Культивирование осуществляли в климатической камере тепла и влажности UT-6070 на различных средах, основанных на сочетании среды Заррука и стерильной озер ной воды в разных соотношениях. Штамм L. fusiformis O9.13F показал наибольшую скорость нарастания фитомассы при температуре культивирования 20 ± 2 °C и интенсивности света 10–30 μмоль фотонов/м2c (режим чередования света и темноты 12:12 ч). Оптимальной средой для культивирования штамма была среда, состоящая из минеральной жидкой среды Заррука и стерилизованной воды из озера в соотношении 5:5. При длительном культивировании (более 20 суток) для предотвращения оседания фосфатов из минеральной среды необходимо применять механическое перемешивание или использовать культиватор КВ-06 Европолитест. Оптимальная частота пересевов культуры – один раз в 5–7 суток. При меньшей частоте пересева отметили, что лимноспира не успевала сформировать полноценные трихомы, и наращивание объема фитомассы замедлялось. Предлагаемая технология культивирования штамма L. fusiformis O9.13F позволяет получать большой объем фитомассы в короткие сроки с незначительными финансовыми затратами. Дальнейшие исследования авторов направлены на изучение возможности использования экологически чистой фитомассы L. fusiformis как самостоятельного биологического компонента в питании человека, а также в качестве функционально значимого рецептурного компонента пищевых продуктов.

Global food shortages make it necessary to look for alternative renewable bioresources. In the south of Western Siberia, the filamentous cyanoprokaryote Limnospira fusiformis triggers seasonal algae bloom in hypergaline alkaline Lake Solenoye. The species has valuable nutritional properties, and its phytomass is a potential source of proteins and biologically active substances. The O9.13F strain of L. fusiformis has a good potential as a bioadditive in animal feeding. The article offers a technology for cultivating O9.13F of L. fusiformis, isolated from Lake Solenoye. The research objectives were to establish the optimal cultivation conditions, medium, and periodicity. The study featured strain O9.13F of filamentous cyanoprokaryote L. fusiformis. The micropipette method made it possible to isolate pure culture from water samples taken from Lake Solenoye, Omsk, Russia, at the end of algae bloom. The cultivation involved a UT-6070 climatic chamber under uniform illumination with light intensity 10–30 μmol photons/m2s and 12-h light-dark circle at 20 ± 2°C on various media: natural habitat – water from the Solenoye Lake; mineral medium – liquid Zarrouk’s medium; agarized Zarrouk’s medium; composite variants, where the ratio of mineral medium vs. water varied from 1:9 to 9:1. Lake water inhibited the culture growth: the trichomes died and sank on day 10–15. Zarrouk’s agarized medium stopped the culture growth as early as on day 2. The most intensive growth and development of the culture was observed in the samples with Zarrouk’s mineral liquid medium and a composite mix of Zarrouk’s medium and sterilized water at a ratio of 5:5. Without stirring, full-fledged trichomes had no time to develop, and the increase in phytomass volume slowed down. O9.13F showed the highest rate of phytomass growth at a cultivation temperature of 20 ± 2°C and a light intensity of 10–30 μmol photons/m2s. The recommended light-dark circle was12:12 h. Zarrouk’s mineral liquid medium and a composite medium of Zarrouk’s medium and sterilized water proved to be optimal in a UT-6070 environmental chamber. Europolitext KV-06 or mechanical mixing could prevent sedimentation of phosphates after 20 days of cultivation. The optimal recultivation frequency was once every 5–7 days. The new cultivation technology made it possible to obtain a significant volume of L. fusiformis phytomass in a short time and with low financial expenses.

 Limnospira fusiformis цианопрокариоты культивирование фитомасса пищевая добавка Западная Сибирь Limnospira fusiformis cyanoprokaryotes cultivation phytomass food additive West ern Siberia Исследование выполнено за счет гранта Российского научного фонда (РНФ) № 22-26-20108, https://rscf.ru/project/22-26-20108 The research was supported by the Russian Science Foundation (RSF), grant No. 22-26-20108, https://rscf.ru/ project/22-26-20108

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