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 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">Dairy industry</journal-id>
   <journal-title-group>
    <journal-title xml:lang="en">Dairy industry</journal-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Молочная промышленность</trans-title>
    </trans-title-group>
   </journal-title-group>
   <issn publication-format="print">1019-8946</issn>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="publisher-id">6a84bda494fbba14f78763f7</article-id>
   <article-id pub-id-type="doi">10.21603/1019-8946-2026-4-95</article-id>
   <article-id pub-id-type="edn">KPCTPI</article-id>
   <article-categories>
    <subj-group subj-group-type="toc-heading" xml:lang="ru">
     <subject>Научная статья</subject>
    </subj-group>
    <subj-group subj-group-type="toc-heading" xml:lang="en">
     <subject>Research Article</subject>
    </subj-group>
    <subj-group>
     <subject>Научная статья</subject>
    </subj-group>
   </article-categories>
   <title-group>
    <article-title xml:lang="en">Effect of Lactose Hydrolysis Stage on Fermented Dairy Beverages</article-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Влияние стадии гидролиза лактозы на качество ферментированных молочных напитков</trans-title>
    </trans-title-group>
   </title-group>
   <contrib-group content-type="authors">
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Каледина</surname>
       <given-names>Марина Васильевна</given-names>
      </name>
      <name xml:lang="en">
       <surname>Kaledina</surname>
       <given-names>Marina Vasil'evna</given-names>
      </name>
     </name-alternatives>
     <email>kaledinamarina@yandex.ru</email>
     <xref ref-type="aff" rid="aff-1"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Витковская</surname>
       <given-names>Виктория Петровна</given-names>
      </name>
      <name xml:lang="en">
       <surname>Vitkovskaya</surname>
       <given-names>Viktoria P.</given-names>
      </name>
     </name-alternatives>
     <bio xml:lang="ru">
      <p>кандидат сельскохозяйственных наук;</p>
     </bio>
     <bio xml:lang="en">
      <p>candidate of agricultural sciences;</p>
     </bio>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Шемякина</surname>
       <given-names>Дарья Александровна</given-names>
      </name>
      <name xml:lang="en">
       <surname>Shemyakina</surname>
       <given-names>Dar'ya Aleksandrovna</given-names>
      </name>
     </name-alternatives>
     <email>litovkina_da@belgau.ru</email>
     <xref ref-type="aff" rid="aff-3"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Поротова</surname>
       <given-names>Елена Юрьевна</given-names>
      </name>
      <name xml:lang="en">
       <surname>Porotova</surname>
       <given-names>Elena Yu.</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-4"/>
    </contrib>
   </contrib-group>
   <aff-alternatives id="aff-1">
    <aff>
     <institution xml:lang="ru">ФГБОУ ВО Белгородский государственный аграрный университет им. В.Я. Горина</institution>
     <city>Белгород</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Belgorod State Agricultural University named after V.Ya. Gorin</institution>
     <city>Belgorod</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-2">
    <aff>
     <institution xml:lang="ru">Белгородский государственный аграрный университет им. В. Я. Горина</institution>
     <city>Майский</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Belgorod State Agricultural University named after V. Gorin</institution>
     <city>Maisky</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-3">
    <aff>
     <institution xml:lang="ru">Белгородский государственный аграрный университет им. В. Я. Горина</institution>
     <city>Белгород</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Belgorod State Agricultural University</institution>
     <city>Belgorod</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-4">
    <aff>
     <institution xml:lang="ru">Крымский федеральный университет им. В. И. Вернадского</institution>
     <city>Симферополь</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Crimean Federal University</institution>
     <city>Simferopol</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <pub-date publication-format="print" date-type="pub" iso-8601-date="2026-08-17T00:00:00+03:00">
    <day>17</day>
    <month>08</month>
    <year>2026</year>
   </pub-date>
   <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2026-08-17T00:00:00+03:00">
    <day>17</day>
    <month>08</month>
    <year>2026</year>
   </pub-date>
   <fpage>58</fpage>
   <lpage>65</lpage>
   <history>
    <date date-type="received" iso-8601-date="2026-03-05T00:00:00+03:00">
     <day>05</day>
     <month>03</month>
     <year>2026</year>
    </date>
    <date date-type="accepted" iso-8601-date="2026-06-09T00:00:00+03:00">
     <day>09</day>
     <month>06</month>
     <year>2026</year>
    </date>
   </history>
   <permissions>
    <copyright-statement xml:lang="ru">© 2026 Каледина М.В., Витковская В.П., Шемякина Д.А., Поротова Е.Ю.</copyright-statement>
    <copyright-statement xml:lang="en">© 2026 Kaledina M.V., Vitkovskaya V.P., Shemyakina D.A., Porotova E.Y.</copyright-statement>
    <copyright-year>2026</copyright-year>
    <copyright-holder xml:lang="ru">Каледина Марина Васильевна, Витковская Виктория Петровна, Шемякина Дарья Александровна, Поротова Елена Юрьевна</copyright-holder>
    <copyright-holder xml:lang="en">Kaledina Marina Vasil'evna, Vitkovskaya Viktoria P., Shemyakina Dar'ya Aleksandrovna, Porotova Elena Yu.</copyright-holder>
   </permissions>
   <self-uri xlink:href="https://moloprom.kemsu.ru/en/nauka/publications/article/6a84bda494fbba14f78763f7/effect-of-lactose-hydrolysis-stage-on-fermented-dairy-beverages/view">https://moloprom.kemsu.ru/en/nauka/publications/article/6a84bda494fbba14f78763f7/effect-of-lactose-hydrolysis-stage-on-fermented-dairy-beverages/view</self-uri>
   <abstract xml:lang="ru">
    <p>В условиях глобальной распространенности лактазной недостаточности и тенденции к снижению потребления добавленных сахаров разработка технологий ферментированных молочных продуктов с гидролизованной лактозой приобретает высокую научно-практическую значимость. Полный или почти полный гидролиз лактозы в кисломолочных продуктах возможен при внесении фермента β-галактозидазы в молоко перед пастеризацией (предварительный гидролиз / прегидролиз) или добавлении лактазы вместе с закваской после пастеризации молока (совместный гидролиз / согидролиз). Цель исследования – сравнительная оценка влияния двух технологических подходов, прегидролиза и согидролиза при внесении ферментного препарата β-галактозидазы NOLA Fit 5500 на биохимические, реологические и органолептические характеристики модельных образцов йогурта и кефира. Установлено, что для термофильной закваски йогурта прегидролиз обеспечивает степень расщепления лактозы 91,7 %, ускорение достижения целевого pH 4,6 на 90 мин и формирование более плотного геля за счет усиленного синтеза экзополисахаридов. Для симбиотической микрофлоры кефира оптимальным является согидролиз, позволяющий достичь максимальной степени гидролиза – 92,2 %, повысить влагоудерживающую способность и снизить синерезис благодаря образованию гидрофильной полимерной сети вокруг казеиновых мицелл. Реологический анализ подтвердил, что прегидролиз способствует повышению вязкости йогуртового сгустка, а согидролиз улучшает стабильность структуры кефира при хранении. Органолептическая оценка показала улучшение вкусового профиля гидролизованных образцов за счет естественной сладости глюкозы и галактозы, что позволяет существенно минимизировать использование сахарозы и других подсластителей. Выбор стадии внесения фермента является критическим параметром, определяющим качество низколактозных и безлактозных ферментированных напитков и открывающим возможности целенаправленного моделирования их функционально-технологических свойств.</p>
   </abstract>
   <trans-abstract xml:lang="en">
    <p>Global lactase deficiency and the trend toward lower consumption of added sugars require new technologies for fermented dairy products with hydrolyzed lactose. Adding β-galactosidase to milk before pasteurization (pre-hydrolysis) or lactase with a starter culture after milk pasteurization (co-hydrolysis) provides complete or nearly complete hydrolysis of lactose in fermented dairy products. Two technological approaches, pre-hydrolysis and co-hydrolysis, were combined with the NOLA Fit 5500 β-galactosidase enzyme preparation. The authors studied their effect on the biochemical, rheological, and sensory profiles of model yogurt and kefir samples. Pre-hydrolysis provided a 91.7% lactose degradation rate for thermophilic yogurt starter cultures, accelerated the achievement of the target pH of 4.6 by 90 min, and formed a denser gel due to enhanced exopolysaccharide synthesis. Co-hydrolysis proved optimal for the symbiotic microflora of kefir: it achieved the highest hydrolysis rate of 92.2%, increased the waterholding capacity, and reduced syneresis by forming a hydrophilic polymer network around casein micelles. The rheological analysis confirmed that pre-hydrolysis increased the viscosity of the yogurt curd, while co-hydrolysis improved the stability of the kefir structure during storage. The sensory assessment revealed an improved flavor profile for the hydrolyzed samples: the natural sweetness of glucose and galactose significantly minimized the need for sucrose and other sweeteners. The enzyme addition stage proved to be a critical parameter determining the quality of lowlactose and lactose-free fermented beverages, opening opportunities for targeted modeling of their functional and technological profiles.</p>
   </trans-abstract>
   <kwd-group xml:lang="ru">
    <kwd>согидролиз</kwd>
    <kwd>прегидролиз</kwd>
    <kwd>β-галактозидаза</kwd>
    <kwd>сквашивание</kwd>
    <kwd>лактоза</kwd>
    <kwd>йогурт</kwd>
    <kwd>кефир</kwd>
    <kwd>экзополисахариды</kwd>
   </kwd-group>
   <kwd-group xml:lang="en">
    <kwd>co-hydrolysis</kwd>
    <kwd>pre-hydrolysis</kwd>
    <kwd>β-galactosidase</kwd>
    <kwd>fermentation</kwd>
    <kwd>lactose</kwd>
    <kwd>yogurt</kwd>
    <kwd>kefir</kwd>
    <kwd>exopolysaccharides</kwd>
   </kwd-group>
  </article-meta>
 </front>
 <body>
  <p></p>
 </body>
 <back>
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