The current paper presents the results of a comparative evaluation of 74 samples of the world collection preserved at the Vavilov Research Institute of Plant Industry named in 2013-2016. The analyses features individual elements of productivity, yield and the variation degree (Cv, %). The samples of the six-rowed barley of Leningradskiy and Potra cultivars have been found early-ripening (vegetation period 68-69 days). The highest capacity for productive tillering (1.93-2.40 productive stems) have been registered in the cultivars of Rupal, Corona, Malva, Chelyabinets 2, Nutans 302, Belgorodets and Talovsky 9. The best graininess of the ear belongs to the following cultivars: 18/7, Tduneja, Spratt, Sv. 66905, Milns Golden Promise, Vladimir. According to the mass of 1000 grains (40.0-52.3 g), T-12 (Nutans 129), Yastreb, Belgorodets, Talovsky 9, Yak-401, Nudum 95, Codac, Vakula, Potra, Kolchan. The best grain mass from one plant (1.40-1.79 g) was registered in 18/7, Tduneja, T-12 (Nutans 129), Elf, Belgorodets, Talovsky 9, Heimdal, Nudum 95, Codac, Vakula and Kolchan. According to the productivity and resistance to lodging, the following samples proved to be stable during the research period: M 1913/88 (Czechoslovakia), Asem (Kazakhstan), Etienne (Canada). Their yield reached 555.0-575.0 g/m2, which is 8.2-12.1 % higher than that of the standard Acha cultivar. The barley samples studied in the research are of practical interest for solving regional breeding problems in Eastern Siberia.