Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 97492 10.21603/2308-4057-2026-1-664 Research Article Research Article Research Article Antilithiasic and antioxidant activities of propolis and bee pollen extracts: In vitro and in silico studies Antilithiasic and antioxidant activities of propolis and bee pollen extracts: In vitro and in silico studies https://orcid.org/0000-0002-6693-7942 Ali Haimoud Safia Ali Haimoud Safia s.alihaimoud@univ-chlef.dz https://orcid.org/0000-0002-3160-5550 Arioui Fatiha Arioui Fatiha https://orcid.org/0000-0002-7805-6499 Medjekane Meriem Medjekane Meriem https://orcid.org/0009-0004-2819-9178 Ali Haimoud Leila Ali Haimoud Leila https://orcid.org/0009-0003-0873-8632 Bahri Khelifa Bahri Khelifa https://orcid.org/0009-0004-0022-6322 Cheikh Asma Cheikh Asma https://orcid.org/0009-0008-3658-4948 Ghiboub Saadia Ghiboub Saadia https://orcid.org/0009-0008-1662-5765 Benabed Hanane Benabed Hanane https://orcid.org/0009-0003-6731-6894 Boudali Ikram Boudali Ikram https://orcid.org/0009-0008-7483-6069 Ayache Besma Ayache Besma https://orcid.org/0009-0008-6906-5490 Beskri Chaima Beskri Chaima Hassiba Benbouali University of Chlef Chlef Алжир Hassiba Benbouali University of Chlef Chlef Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria University Center Nour Bachir El Bayadh Алжир University Center Nour Bachir El Bayadh Algeria Public Hospital Chlef Алжир Public Hospital Chlef Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria Hassiba Benbouali University of Chlef Ouled Fares Алжир Hassiba Benbouali University of Chlef Ouled Fares Algeria 15 04 2025 15 04 2025 14 1 138 147 24 05 2024 05 11 2024 https://jfrm.ru/en/issues/23173/23425/

Urolithiasis is a wide-spread condition with no efficient pharmacological treatments. It causes the formation of renal stones. The article describes the antioxidant and anti-lithiasis potencies of extracts of Algerian propolis and bee pollen. Their inhibiting effect on calcium oxalate crystallization was assessed against citric acid using UV-Visible spectrophotometry at 620 nm. The effect of rutin and liquiritin on xanthine dehydrogenase was analyzed in silico. The highest levels (p ˂ 0.05) of total phenols (129.28 ± 1.51 mg GAE/g) and flavonoids (77.58 ± 1.95 mg QEQ/g) belonged to the bee pollen ethanolic extract. The same extract had the strongest (p < 0.05) DPPH scavenging capacity (9.420 ± 0.002 μg/mL). The ethanolic extract of propolis possessed the highest antioxidant potency (63.05 ± 3.49%) according to the β-carotene assay. Crystal nucleation in the presence of 16 mg/mL extracts was the same as in the samples tested with citric acid. The propolis ethanolic extract (82.83 ± 0.83%) demonstrated the maximal inhibition of crystal growth at 16 mg/mL, compared with citric acid (91.90 ± 0.26%). Based on the binding free energy (ΔG) and root mean square deviation, the ligands were efficient inhibitors of xanthine dehydrogenase. Bee pollen and propolis proved to contain bioactive components that can make them an alternative to traditional methods of urolithiasis prevention and treatment.

Urolithiasis is a wide-spread condition with no efficient pharmacological treatments. It causes the formation of renal stones. The article describes the antioxidant and anti-lithiasis potencies of extracts of Algerian propolis and bee pollen. Their inhibiting effect on calcium oxalate crystallization was assessed against citric acid using UV-Visible spectrophotometry at 620 nm. The effect of rutin and liquiritin on xanthine dehydrogenase was analyzed in silico. The highest levels (p ˂ 0.05) of total phenols (129.28 ± 1.51 mg GAE/g) and flavonoids (77.58 ± 1.95 mg QEQ/g) belonged to the bee pollen ethanolic extract. The same extract had the strongest (p < 0.05) DPPH scavenging capacity (9.420 ± 0.002 μg/mL). The ethanolic extract of propolis possessed the highest antioxidant potency (63.05 ± 3.49%) according to the β-carotene assay. Crystal nucleation in the presence of 16 mg/mL extracts was the same as in the samples tested with citric acid. The propolis ethanolic extract (82.83 ± 0.83%) demonstrated the maximal inhibition of crystal growth at 16 mg/mL, compared with citric acid (91.90 ± 0.26%). Based on the binding free energy (ΔG) and root mean square deviation, the ligands were efficient inhibitors of xanthine dehydrogenase. Bee pollen and propolis proved to contain bioactive components that can make them an alternative to traditional methods of urolithiasis prevention and treatment.

 Bee products calcium oxalate crystallization extracts DPPH method antilithiasic activity xanthine dehydrogenase Bee products calcium oxalate crystallization extracts DPPH method antilithiasic activity xanthine dehydrogenase

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