Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 92589 10.21603/2074-9414-2024-4-2549 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Collagen Hydrolysed from Maral Raw Material: Production Technology and Biochemical Composition Коллаген, гидролизованный из сырья маралов: технология получения и биохимический состав https://orcid.org/0000-0001-7878-8529 Кротова Мария Георгиевна Krotova Maria G. otdel_wniipo@mail.ru

кандидат сельскохозяйственных наук;

candidate of agricultural sciences;

 https://orcid.org/0000-0002-5172-4143 Гришаева Ирина Николаевна Grishaeva Irina N. otdel_wniipo@mail.ru

кандидат биологических наук;

candidate of sciences in biology;

 Федеральный Алтайский научный центр агробиотехнологий Барнаул Россия Federal Altai Scientific Center for Agrobiotechnology Barnaul Russian Federation Федеральный Алтайский научный центр агробиотехнологий Барнаул Россия Federal Altai Scientific Center for Agrobiotechnology Barnaul Russian Federation 24 12 2024 24 12 2024 54 4 884 896 13 03 2024 04 06 2024 https://fptt.ru/en/issues/23109/23164/

Коллаген получает все большее признание как составная часть лечебного питания благодаря многостороннему и благоприятному действию на организм человека. Среди огромного разнообразия источников коллагена особое место занимает сырье пантовых оленей, которое более 20 веков используется в традиционной китайской медицине для поддержания здоровья, в том числе костно-мышечной системы. Цель исследования – провести анализ биохимического состава сырья маралов и гидролизованного коллагена в зависимости от технологии получения. Объектами исследования являлись шкуры и сухожилия 10 маралов. Сырье измельчали до фарша и гидролизовали. Массовую долю коллагена определяли по концентрации оксипролина. Расчет выхода сухих веществ осуществляли по ГОСТ 31640-2012. Определение массовой концентрации макро- и микроэлементов проводили методом атомно-адсорбционной спектроскопии. Аминный азот определяли формольным титрованием. Аминокислотный состав исследовали с применением высокоэффективной жидкостной хроматографии. В результате исследований определили аминокислотный состав коллагена. Среди превалирующих аминокислот отмечены глицин – 14,36 г/100 г, пролин – 8,87 г/100 г и оксипролин – 7,83 г/100 г. Выявили различия в концентрации аминокислот в зависимости от технологии получения. При ферментации отметили увеличение содержания аргинина и лизина в 4–5 раз, а при высокотемпературной экстракции – оксипролина, глутаминовой кислоты и треонина в 1,5–2,3 раза. Показали, что для получения коллагена с максимальным выходом сухих веществ и количеством аминокислот необходимо проводить поэтапный гидролиз, включающий ферментацию и высокотемпературную экстракцию. Коллаген, гидролизованный из сырья маралов, содержал значительное количество глицина, пролина и оксипролина, что делает перспективным его дальнейшее применение для дополнения продуктов, лимитированных по данным аминокислотам. Необходимо проведение дальнейших исследований влияния гидролизованного коллагена на организм человека.

Collagen has a complex beneficial effect on human health, which makes it a popular component in various therapeutic diets. Deer antlers are a promising source of collagen. It has been used in traditional Chinese medicine for more than 20 centuries as an additive that supports the musculoskeletal system. The article describes the effect of extraction technology on the amino acid and biochemical composition of collagen obtained from the Altai wapiti, or maral (Cervus Canadensis). The research featured hydrolysates obtained from ground skin and tendons of ten marals. The mass fraction of collagen was determined by the concentration of oxyproline. The yield of dry solids was calculated in line with State Standard GOST 316402012. The method of atomic adsorption spectroscopy made it possible to calculate the mass concentration of macro- and microelements. Amine nitrogen was detected by formol titration while the general amino acid composition was studied using the method of high-performance liquid chromatography. The list of amino acids included glycine (14.36 g/100g), proline (8.87 g/100g), and oxyproline (7.83 g/100 g). Their concentration depended on the production technology. The content of arginine and lysine increased 4–5 times during fermentation and 1.5–2.3 times during high-temperature extraction of oxyproline, glutamic acid, and threonine. A step-by-step hydrolysis protocol with fermentation and high-temperature extraction provided the maximal yield of dry solids and amino acids. In this study, the collagen hydrolyzed from maral skin and tendons was rich in glycine, proline, and oxyproline, which makes it a prospective additive to be used in products that lack these amino acids. The effect of hydrolyzed maral collagen on the human body needs further research

 Марал коллаген шкура сухожилия гидролиз аминокислотный состав ферментация зольные компоненты Maral collagen skin tendons hydrolysis amino acid composition fermentation ash components Работа выполнена в рамках проекта с использованием мер государственной поддержки за счет средств гранта Управления Алтайского края по развитию туризма и курортной деятельности по теме «Проведение научных исследований по изучению природных лечебных ресурсов региона и разработке методик их применения и сохранения, выявлению перспективных территорий для развития санаторно-курортной отрасли в Алтайском крае» (договор 9-2022 от 30.11.2022) и при финансовой поддержке Министерства науки и высшего образования Российской Федерации (Минобрнауки России) в Федеральном Алтайском научном центре агробиотехнологий. The research was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement 9-2022, November 30, 2022): Natural therapeutic resources of the Altai Region: application and preservation; Development prospects of health resort industry in the Altai Region.

Sun S, Gao Y, Chen J, Liu R. Identification and release kinetics of peptides from tilapia skin collagen during alcalase hydrolysis. Food Chemistry. 2022;378:132089. https://doi.org/10.1016/j.foodchem.2022.132089 Sun S, Gao Y, Chen J, Liu R. Identification and release kinetics of peptides from tilapia skin collagen during alcalase hydrolysis. Food Chemistry. 2022;378:132089. https://doi.org/10.1016/j.foodchem.2022.132089 Nuñez SM, Guzmán F, Valencia P, Almonacid S, Cárdenas C. Collagen as a source of bioactive peptides: A bioinformatics approach. Electronic Journal of Biotechnology. 2020;48:101–108. https://doi.org/10.1016/j.ejbt.2020.09.009 Nuñez SM, Guzmán F, Valencia P, Almonacid S, Cárdenas C. Collagen as a source of bioactive peptides: A bioinformatics approach. Electronic Journal of Biotechnology. 2020;48:101–108. https://doi.org/10.1016/j.ejbt.2020.09.009 Palamutoğlu R, Palamutoğlu MI. Beneficial health effects of collagen hydrolysates. Studies in natural products. 2024;80:477–503. https://doi.org/10.1016/B978-0-443-15589-5.00014-1 Palamutoğlu R, Palamutoğlu MI. Beneficial health effects of collagen hydrolysates. Studies in natural products. 2024;80:477–503. https://doi.org/10.1016/B978-0-443-15589-5.00014-1 Wang H. A Review of the Effects of Collagen Treatment in Clinical Studies. Polymers. 2021;13(22):3868. https:// doi.org/10.3390/polym13223868 Wang H. A Review of the Effects of Collagen Treatment in Clinical Studies. Polymers. 2021;13(22):3868. https:// doi.org/10.3390/polym13223868 Shavlovskaya OA, Bokova IA, Romanov ID, Shavlovsky NI. Efficacy of undenatured and hydrolyzed type II collagen in the treatment of pain syndrome. Russian Medical Inquiry. 2022;6(10):571–575. (In Russ.). https://doi.org/10.32364/2587- 6821-2022-6-10-571-575 Shavlovskaya OA, Bokova IA, Romanov ID, Shavlovsky NI. Efficacy of undenatured and hydrolyzed type II collagen in the treatment of pain syndrome. Russian Medical Inquiry. 2022;6(10):571–575. (In Russ.). https://doi.org/10.32364/2587- 6821-2022-6-10-571-575 Gromova OA, Torshin IYu, Lila AM, Shavlovskaya OA. On the prospects for the use of undenatured type II collagen in the treatment of osteoarthritis and other joint diseases. Modern Rheumatology Journal. 2022;16(4):111–116. http://doi.org/ 10.14412/1996-7012-2022-4-111-116 Gromova OA, Torshin IYu, Lila AM, Shavlovskaya OA. On the prospects for the use of undenatured type II collagen in the treatment of osteoarthritis and other joint diseases. Modern Rheumatology Journal. 2022;16(4):111–116. http://doi.org/ 10.14412/1996-7012-2022-4-111-116 Oslan SNH, Li CX, Shapawi R, Mokhtar RAM, Noordin WN, Huda N. Extraction and Characterization of Bioactive Fish By-Product Collagen as Promising for Potential Wound Healing agent in Pharmaceutical Applications: Current Trend and Future Perspective. International of Food Science. 2022;9437878. https://doi.org/10.1155/2022/9437878 Oslan SNH, Li CX, Shapawi R, Mokhtar RAM, Noordin WN, Huda N. Extraction and Characterization of Bioactive Fish By-Product Collagen as Promising for Potential Wound Healing agent in Pharmaceutical Applications: Current Trend and Future Perspective. International of Food Science. 2022;9437878. https://doi.org/10.1155/2022/9437878 Xu R, Wu J, Zheng L, Zhao M. Undenatured type II collagen and its role in improving osteoarthritis. Ageing Research Reviews. 2023;91:102080. https://doi.org/10.1016/j.arr.2023.102080 Xu R, Wu J, Zheng L, Zhao M. Undenatured type II collagen and its role in improving osteoarthritis. Ageing Research Reviews. 2023;91:102080. https://doi.org/10.1016/j.arr.2023.102080 Kıyak BD, Çınkır NI, Çelebi Y, Malçok SD, Koç GC, Adal S, Yüksel AN, et al. Advanced technologies for the collagen extraction from food waste – A review on recent progress. Microchemical Journal. 2024;201:110404. https://doi.org/ 10.1016/j.microc.2024.110404 Kıyak BD, Çınkır NI, Çelebi Y, Malçok SD, Koç GC, Adal S, Yüksel AN, et al. Advanced technologies for the collagen extraction from food waste – A review on recent progress. Microchemical Journal. 2024;201:110404. https://doi.org/ 10.1016/j.microc.2024.110404 Gao R, Yu Q, Shen Y, Chu Q, Chen G, Fenet S, et al. Production, bioactive properties, and potential applications of fish protein hydrolysates: Developments and challenges. Trends in Food Science and technology. 2021;110:687–699. https:// doi.org/10.1016/j.tifs.2021.02.031 Gao R, Yu Q, Shen Y, Chu Q, Chen G, Fenet S, et al. Production, bioactive properties, and potential applications of fish protein hydrolysates: Developments and challenges. Trends in Food Science and technology. 2021;110:687–699. https:// doi.org/10.1016/j.tifs.2021.02.031 Du B, Deng G, Zaman F, Ma H, Li X, Chen J, et al. Huang Antioxidant cuttlefish collagen hydrolysate against ethyl carbamate-induced oxidative damage. Royal Society of Chemistry. 2021;11:2337–2345. https://doi.org/10.1039/d0ra08487e Du B, Deng G, Zaman F, Ma H, Li X, Chen J, et al. Huang Antioxidant cuttlefish collagen hydrolysate against ethyl carbamate-induced oxidative damage. Royal Society of Chemistry. 2021;11:2337–2345. https://doi.org/10.1039/d0ra08487e Shikh EV. Clinical and pharmacological aspects of application of hydrolyzed collagen of the second type for prevention and treatment of osteoarthrosis. Pharmacology and Pharmacotherapy. 2021;(4):10–18. (In Russ.). https://doi.org/ 10.46393/2713-2129_2021_4_10_18. https://elibrary.ru/SNKUBE Shikh EV. Clinical and pharmacological aspects of application of hydrolyzed collagen of the second type for prevention and treatment of osteoarthrosis. Pharmacology and Pharmacotherapy. 2021;(4):10–18. (In Russ.). https://doi.org/ 10.46393/2713-2129_2021_4_10_18. https://elibrary.ru/SNKUBE Guo Z, Yi D, Hu B, Zhu L, Zhang J, Yang Y, et al. Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice. Food Science and Human Wellness. 2023;12(5):1637–1645. http://doi.org/10.1016/j.fshw.2023.02.017 Guo Z, Yi D, Hu B, Zhu L, Zhang J, Yang Y, et al. Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice. Food Science and Human Wellness. 2023;12(5):1637–1645. http://doi.org/10.1016/j.fshw.2023.02.017 González-Noriega JA, Valenzuela-Melendres M, Hernández–Mendoza A, Astiazarán-García H, Ángel Mazorra- Manzano M, Peña-Ramos EA. Hydrolysates and peptide fractions from pork and chicken skin collagen as pancreatic lipase inhibitors. Food Chemistry:X. 2022;13:100247. http://doi.org/10.1016/j.fochx.2022.100247 González-Noriega JA, Valenzuela-Melendres M, Hernández–Mendoza A, Astiazarán-García H, Ángel Mazorra- Manzano M, Peña-Ramos EA. Hydrolysates and peptide fractions from pork and chicken skin collagen as pancreatic lipase inhibitors. Food Chemistry:X. 2022;13:100247. http://doi.org/10.1016/j.fochx.2022.100247 Qi L, Zhang H, Guo Y, Zhang C, Xu Y. A novel calcium-binding peptide from bovine bone collagen hydrolysate and chelation mechanism and calcium absorption activity of peptide-calcium chelate. Food Chemistry. 2023;410:135387. https:// doi.org/10.1016/j.foodchem.2023.135387 Qi L, Zhang H, Guo Y, Zhang C, Xu Y. A novel calcium-binding peptide from bovine bone collagen hydrolysate and chelation mechanism and calcium absorption activity of peptide-calcium chelate. Food Chemistry. 2023;410:135387. https:// doi.org/10.1016/j.foodchem.2023.135387 Li B, Chen F, Wang X, Ji B, Wu Y. Isolation and identification of antioxidative peptides from porcine collagen hydrolysat by conescutive xhromatography and electrospray ionization-mass spectrometry. Food Chemistry. 2007;102(4):1135–1143. https://doi.org/10.1016/j.foodchem.2006.07.002 Li B, Chen F, Wang X, Ji B, Wu Y. Isolation and identification of antioxidative peptides from porcine collagen hydrolysat by conescutive xhromatography and electrospray ionization-mass spectrometry. Food Chemistry. 2007;102(4):1135–1143. https://doi.org/10.1016/j.foodchem.2006.07.002 Wang H, Tu Z, Wang H. Preparation of high content collagen peptides and study of their biological activities. Food Research International. 2023;174:113561. https://doi.org/10.1016/j.foodres.2023.113561 Wang H, Tu Z, Wang H. Preparation of high content collagen peptides and study of their biological activities. Food Research International. 2023;174:113561. https://doi.org/10.1016/j.foodres.2023.113561 Espinales C, Romero-Pena M, Calderon G, Vergara K, Caceres PJ, Castillo P. Collagen, protein hydrolysates and chitin from by-products of fish and shellfish: An overview. Heliyon. 2023;9(4):e14937. https://doi.org/10.1016/j.heliyon.2023.e14937 Espinales C, Romero-Pena M, Calderon G, Vergara K, Caceres PJ, Castillo P. Collagen, protein hydrolysates and chitin from by-products of fish and shellfish: An overview. Heliyon. 2023;9(4):e14937. https://doi.org/10.1016/j.heliyon.2023.e14937 Zhou Z. Zhao D, Zhang P, Zhang M, Leng X, Yao B. The enzymatic hydrolysates from deer sinew promote MC3T3-E1 cell proliferation and extracellular matrix synthesis by regulating multiple functional genes. BMC Complementary Medicine and Therapies. 2021;21:59. https://doi.org/10.1186/s12906-021-03240-2 Zhou Z. Zhao D, Zhang P, Zhang M, Leng X, Yao B. The enzymatic hydrolysates from deer sinew promote MC3T3-E1 cell proliferation and extracellular matrix synthesis by regulating multiple functional genes. BMC Complementary Medicine and Therapies. 2021;21:59. https://doi.org/10.1186/s12906-021-03240-2 Zhang H, Dong Y, Qi B, Liu L, Zhou G, Bai X, et al. Preventive effects of collagen peptide from deer sinew on bone loss in ovariectomized rats. Evidence-Based Complementary and Alternative Medicine. 2014;2014(1):627285. https:// doi.org/10.1155/2014/627285 Zhang H, Dong Y, Qi B, Liu L, Zhou G, Bai X, et al. Preventive effects of collagen peptide from deer sinew on bone loss in ovariectomized rats. Evidence-Based Complementary and Alternative Medicine. 2014;2014(1):627285. https:// doi.org/10.1155/2014/627285 Zhang H, Zhao Y, Li YQ, Sun XD. Bai XY, Zhao DQ. Effects of deer tendons collagen on osteoporosis rats induced by retinoic acid. Zhong Yao Cai. 2010;33(3):411–414. Zhang H, Zhao Y, Li YQ, Sun XD. Bai XY, Zhao DQ. Effects of deer tendons collagen on osteoporosis rats induced by retinoic acid. Zhong Yao Cai. 2010;33(3):411–414. Wang I-L, Hsiao C-Y, Shen J, Wang Y, Huang C-C, Chen Y-M. The effects of Jilin sika Deer’s (Cervus dybowski) tendon liquid supplementation on endurance drop jumps performance, biochemistry profile of free boxing players. Journal Ethnopharmacology. 2019;245:112119. https://doi.org/10.1016/j.jep.2019.112119 Wang I-L, Hsiao C-Y, Shen J, Wang Y, Huang C-C, Chen Y-M. The effects of Jilin sika Deer’s (Cervus dybowski) tendon liquid supplementation on endurance drop jumps performance, biochemistry profile of free boxing players. Journal Ethnopharmacology. 2019;245:112119. https://doi.org/10.1016/j.jep.2019.112119 Xu X, Wang D, Li J, Zeng X, Zhang Z, Zhu J, et.al. Collagen hydrolysates from deer tendon: Preparation assisted with different ultrasound pretreatment times and promotion in MC3T3-E1 cell proliferation and antioxidant activities. Process Biochemistry. 2023;133:228–240. https://doi.org/10.1016/j.procbio.2023.09.010 Xu X, Wang D, Li J, Zeng X, Zhang Z, Zhu J, et.al. Collagen hydrolysates from deer tendon: Preparation assisted with different ultrasound pretreatment times and promotion in MC3T3-E1 cell proliferation and antioxidant activities. Process Biochemistry. 2023;133:228–240. https://doi.org/10.1016/j.procbio.2023.09.010 Krotova MG, Lunitsyn VG. Effectiveness of using enzymes of microbial origin in maral raw stuff processing. Siberian Herald of Agricultural Science. 2017;47(5):97–103. (In Russ.). https://doi.org/10.26898/0370-8799-2017-5-12; https:// elibrary.ru/TLOWWE Krotova MG, Lunitsyn VG. Effectiveness of using enzymes of microbial origin in maral raw stuff processing. Siberian Herald of Agricultural Science. 2017;47(5):97–103. (In Russ.). https://doi.org/10.26898/0370-8799-2017-5-12; https:// elibrary.ru/TLOWWE Krotova MG. Improving the technology of hydrolysis of marals’ raw materials. Bulletin of KSAU. 2020;(5):147–152. (In Russ.). https://doi.org/10.36718/1819-4036-2020-5-147-152; https://elibrary.ru/BABKSV Krotova MG. Improving the technology of hydrolysis of marals’ raw materials. Bulletin of KSAU. 2020;(5):147–152. (In Russ.). https://doi.org/10.36718/1819-4036-2020-5-147-152; https://elibrary.ru/BABKSV Goto S, Nagao K, Bannai M, Takahashi M, Nakahara K, Kangawa K, et al. Anorexia in rats caused by a valinedeficient diet is not ameliorated by systemic ghrelin treatment. Neuroscience. 2010;166(1):333–340. https://doi.org/10.1016/ j.neuroscience.2009.12.013 Goto S, Nagao K, Bannai M, Takahashi M, Nakahara K, Kangawa K, et al. Anorexia in rats caused by a valinedeficient diet is not ameliorated by systemic ghrelin treatment. Neuroscience. 2010;166(1):333–340. https://doi.org/10.1016/ j.neuroscience.2009.12.013 Worlanyo НG, Jiang S, Yu Y, Liu B, Zhou Q, Sun C, et.al. Effects of dietary threonine on growth and immune response of oriental river prawn (Macrobrachium nipponense). Fish and Shellfish Immunology. 2022;128:288–299. https:// doi.org/10.1016/j.fsi.2022.07.072 Worlanyo HG, Jiang S, Yu Y, Liu B, Zhou Q, Sun C, et.al. Effects of dietary threonine on growth and immune response of oriental river prawn (Macrobrachium nipponense). Fish and Shellfish Immunology. 2022;128:288–299. https:// doi.org/10.1016/j.fsi.2022.07.072 Chanmangkang S, Maneerote J, Surayot U, Panya A, You S, Wangtueai S. Physicochemical and biological properties of collagens obtained from tuna tendon by using the ultrasound-assisted extraction. Journal of Agriculture and Food Research. 2024;15:100984. https://doi.org/10.1016/j.jafr.2024.100984 Chanmangkang S, Maneerote J, Surayot U, Panya A, You S, Wangtueai S. Physicochemical and biological properties of collagens obtained from tuna tendon by using the ultrasound-assisted extraction. Journal of Agriculture and Food Research. 2024;15:100984. https://doi.org/10.1016/j.jafr.2024.100984 Guo H, Zhang Q, Liu X, Zhang H, Wang S. Wen X, et al. Dietary hydroxyproline promotes collagen deposition in swim bladder through regulating biosynthesis of amino acid: In-vitro and in-vivo investigations in Nibea coibor. Aquaculture. 2023;573:739614. https://doi.org/10.1016/j.aquaculture.2023.739614 Guo H, Zhang Q, Liu X, Zhang H, Wang S. Wen X, et al. Dietary hydroxyproline promotes collagen deposition in swim bladder through regulating biosynthesis of amino acid: In-vitro and in-vivo investigations in Nibea coibor. Aquaculture. 2023;573:739614. https://doi.org/10.1016/j.aquaculture.2023.739614 Sun, T, Qiang S, Lu C, Xu F. Composition-dependent energetic contribution of complex salt bridges to collagen stability. Biophysical Journal. 2021;120:3429–3436. https://doi.org/10.1016/j.bpj.2021.05.028 Sun, T, Qiang S, Lu C, Xu F. Composition-dependent energetic contribution of complex salt bridges to collagen stability. Biophysical Journal. 2021;120:3429–3436. https://doi.org/10.1016/j.bpj.2021.05.028 Yu C-H, Khare E, Narayan OP, Parker R, Kaplan DL, Buehler J. ColаGen: An end-to-end deep learning model to predict thermal stability of de novo collagen sequences. Journal of the Mechanical Behavior of Biomedical Materials. 2022; 125:104921. https://doi.org/10.1016/j.jmbbm.2021.104921 Yu C-H, Khare E, Narayan OP, Parker R, Kaplan DL, Buehler J. ColaGen: An end-to-end deep learning model to predict thermal stability of de novo collagen sequences. Journal of the Mechanical Behavior of Biomedical Materials. 2022; 125:104921. https://doi.org/10.1016/j.jmbbm.2021.104921 Petcharat T, Benjakul S, Karnjanapratum S, Nalinanon S. Ultrasound-assisted extraction of collagen from clown featherback (Chitala ornata) skin: yield and molecular characteristics. Journal of the Science of Food and Agriculture. 2021; 101(2):648–658. https://doi.org/10.1002/jsfa.10677 Petcharat T, Benjakul S, Karnjanapratum S, Nalinanon S. Ultrasound-assisted extraction of collagen from clown featherback (Chitala ornata) skin: yield and molecular characteristics. Journal of the Science of Food and Agriculture. 2021; 101(2):648–658. https://doi.org/10.1002/jsfa.10677 Текеев А. А. Значение коллагена в биологической ценности мяса // Гигиена питания. 1997. Т. 2. С. 16–19. Tekeev AA. The importance of collagen in the biological value of meat. Hygiene and Sanitation. 1997;2:16–19. (In Russ.). Yaremenko OB, Anokhina HA, Burianov OA. Joint. Cartilage. Collagen. Injury. 2020;21(4):6–12. (In Russ.). https:// doi.org/10.22141/1608-1706.4.21.2020.212531; https://elibrary.ru/BJFWDU Yaremenko OB, Anokhina HA, Burianov OA. Joint. Cartilage. Collagen. Injury. 2020;21(4):6–12. (In Russ.). https:// doi.org/10.22141/1608-1706.4.21.2020.212531; https://elibrary.ru/BJFWDU Hernández-Ruiz KL, López-Cervantes J, Sánchez-Machado DI, Campas-Baypoli ON, Quintero-Guerrero AA, Grijalva-Delgado MdeL. Collagen peptide fractions from tilapia (Oreochromis aureus Steindachner, 1864) scales: Chemical characterization and biological activity. Food Bioscience. 2023;53:102658. https://doi.org/10.1016/j.fbio.2023.102658 Hernández-Ruiz KL, López-Cervantes J, Sánchez-Machado DI, Campas-Baypoli ON, Quintero-Guerrero AA, Grijalva-Delgado MdeL. Collagen peptide fractions from tilapia (Oreochromis aureus Steindachner, 1864) scales: Chemical characterization and biological activity. Food Bioscience. 2023;53:102658. https://doi.org/10.1016/j.fbio.2023.102658 Guo L, Harnedy PA, Li B, Hou H, Zhang Z, Zhao X, et al. Food protein-derived chelating peptides: biofunctional ingredients for dietary mineral bioavailability enhancement. Trends Food Science and Technology. 2014;37(2):92–105. https:// doi.org/10.1016/j.tifs.2014.02.007 Guo L, Harnedy PA, Li B, Hou H, Zhang Z, Zhao X, et al. Food protein-derived chelating peptides: biofunctional ingredients for dietary mineral bioavailability enhancement. Trends Food Science and Technology. 2014;37(2):92–105. https:// doi.org/10.1016/j.tifs.2014.02.007 Lee SH, Song KB. Isolation of a calcium-binding peptide from enzymatic hydrolysates of porcine blood plasma protein. Journal of the Korean Society for Applied Biological Chemistry. 2009;52:290–294. https://doi.org/10.3839/ jksabc.2009.051 Lee SH, Song KB. Isolation of a calcium-binding peptide from enzymatic hydrolysates of porcine blood plasma protein. Journal of the Korean Society for Applied Biological Chemistry. 2009;52:290–294. https://doi.org/10.3839/ jksabc.2009.051 Lee HS, Oh KJ, Moon YW, In Y, Lee HJ, Kwon SY. Intra-articular injection of type I Atelocollagen to alleviate knee pain: a double-blind, randomized controlled trial. Cartilage. 2021;13(1):342–350. https://doi.org/10.1177/1947603519865304 Lee HS, Oh KJ, Moon YW, In Y, Lee HJ, Kwon SY. Intra-articular injection of type I Atelocollagen to alleviate knee pain: a double-blind, randomized controlled trial. Cartilage. 2021;13(1):342–350. https://doi.org/10.1177/1947603519865304 Ren B, Yue K, Zhang Y, Fu Y. Collagen-derived peptides as prebiotics to improve gut health. Current Opinion in Food Science. 2024;55:101123. https://doi.org/10.1016/j.cofs.2024.101123 Ren B, Yue K, Zhang Y, Fu Y. Collagen-derived peptides as prebiotics to improve gut health. Current Opinion in Food Science. 2024;55:101123. https://doi.org/10.1016/j.cofs.2024.101123 Ahmed M, Verma AK, Patel R. Collagen extraction and recent biological activities of collagen peptides derived from sea-food waste: A review. Sustainable Chemistry and Pharmacy. 2020;18:100315. https://doi.org/10.1016/j.scp.2020.100315 Ahmed M, Verma AK, Patel R. Collagen extraction and recent biological activities of collagen peptides derived from sea-food waste: A review. Sustainable Chemistry and Pharmacy. 2020;18:100315. https://doi.org/10.1016/j.scp.2020.100315 Gharehbeglou P, Sarabandi K, Akbarbaglu Z. Insights into enzymatic hydrolysis: Exploring effects on antioxidant and functional properties of bioactive peptides from Chlorella proteins. Journal of Agriculture and Food Research. 2024;16:101129. https://doi.org/10.1016/j.jafr.2024.101129 Gharehbeglou P, Sarabandi K, Akbarbaglu Z. Insights into enzymatic hydrolysis: Exploring effects on antioxidant and functional properties of bioactive peptides from Chlorella proteins. Journal of Agriculture and Food Research. 2024;16:101129. https://doi.org/10.1016/j.jafr.2024.101129 Mohanty U, Majumdar RK, Mohanty B, Mehta NK, Parhi J. Influence of the extent of enzymatic hydrolysis on the functional properties of protein hydrolysates from visceral waste of Labeo rohita. Food Science Technology. 2021;58(11):4349–4358. https://doi.org/10.1007/s13197-020-04915-3 Mohanty U, Majumdar RK, Mohanty B, Mehta NK, Parhi J. Influence of the extent of enzymatic hydrolysis on the functional properties of protein hydrolysates from visceral waste of Labeo rohita. Food Science Technology. 2021;58(11):4349–4358. https://doi.org/10.1007/s13197-020-04915-3 Bai L, Tian X, Wang Y, Zhang K, Guo J, Ma C, et al. Antioxidant activity during in vitro gastrointestinal digestion and the mode of action with tannins of cowhide-derived collagen hydrolysates: The effects of molecular weight. Food Bioscience. 2023;53:102773. https://doi.org/10.1016/j.fbio.2023.102773 Bai L, Tian X, Wang Y, Zhang K, Guo J, Ma C, et al. Antioxidant activity during in vitro gastrointestinal digestion and the mode of action with tannins of cowhide-derived collagen hydrolysates: The effects of molecular weight. Food Bioscience. 2023;53:102773. https://doi.org/10.1016/j.fbio.2023.102773 Викторова Е. П., Боковикова Т. Н., Лисовая Е. В. Актуальность создания хелатных комплексов биогенных металлов и фосфолипидов для обогащения продуктов питания // Технологии пищевой и перерабатывающей промышленности АПК – продукты здорового питания. 2019. № 2. С. 46–50. Viktorova EP, Bokovikova TN, Lisovaya EV. The relevance of creating chelate complexes of biogenic metals and phospholipids for the enrichment of food products. Technologies of the Food and Processing Industry of the Agro-Industrial Complex-Healthy Food Products. 2019;(2):46–50. (In Russ.).