Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 90284 10.21603/2308-4057-2025-2-647 Research Article Research Article Research Article Barnûf leaves: antioxidant, antimicrobial, antidiabetic, anti-obesity, antithyroid, and anticancer properties Barnûf leaves: antioxidant, antimicrobial, antidiabetic, anti-obesity, antithyroid, and anticancer properties https://orcid.org/0000-0003-2792-6922 Essa Rowida Younis Essa Rowida Younis https://orcid.org/0000-0002-8507-0694 Elsebaie Essam Mohamed Elsebaie Essam Mohamed essam.ahmed@agr.kfs.edu.eg https://orcid.org/0000-0003-0920-6729 Abdelrhman Wesam Mohamed Abdelrhman Wesam Mohamed https://orcid.org/0009-0000-2083-6444 Badr Mohamed Reda Badr Mohamed Reda Kafrelshiekh university Kafr-El-Shiekh Египет Kafrelshiekh university Kafr-El-Shiekh Egypt Kafrelsheikh University Kafr-El-Shiekh Египет Kafrelsheikh University Kafr-El-Shiekh Egypt Al-Azhar University Tanta Египет Al-Azhar University Tanta Egypt Tanta University Tanta Египет Tanta University Tanta Egypt 01 01 2025 01 01 2025 13 2 394 408 01 11 2024 05 03 2024 https://jfrm.ru/en/issues/22898/22996/

Barnûf (Pluchea dioscoridis L.) is a wild plant that grows in Egypt. Barnûf leaves are utilized as a folk medicine, as well as part of food and drink formulations. Their numerous biological benefits include anti-inflammatory and antioxidant properties. We examined the antioxidant, antidiabetic, anti-obesity, antithyroid, and anticancer activities of methanol, ethanol, and acetone extracts of barnûf leaves. The methanol extract exhibited the highest total phenolic (241.50 ± 3.71 mg GAE/g extract) and flavonoid (256.18 ± 3.19 mg QE/g extract) contents. All three extracts proved to possess good antioxidant, antimicrobial, antidiabetic, anti-obesity, antithyroid, and anticancer activities. Ellagic acid was the most abundant phenolic acid in the methanolic (30.33%) and ethanolic (24.71%) extracts. The antioxidant experiments revealed that the methanolic extract had potent DPPH• (IC50 = 18.21 μg/mL) and ABTS•+ (IC50 = 17.6 μg/mL) scavenging properties. The acetone extract demonstrated the highest antimicrobial activity against gramnegative bacteria. Regarding α-amylase and α-glucosidase inhibition, the methanolic extract showed the most potent activity with IC50 values of 104.28 ± 1.97 and 133.76 ± 2.09 μg/mL, respectively. The methanolic extract also proved to be the strongest inhibitor of lipase and thyroid peroxidase, with IC50 values of 127.35 and 211.2 μg/mL, respectively. In addition, the methanolic extract showed the strongest anticancer activity against MCF7-1 and H1299-1 lines with IC50 values of 29.3 and 18.4 μg/mL, respectively. The findings suggest that barnûf leaf extracts could be used in functional foods and pharmaceuticals.

Barnûf (Pluchea dioscoridis L.) is a wild plant that grows in Egypt. Barnûf leaves are utilized as a folk medicine, as well as part of food and drink formulations. Their numerous biological benefits include anti-inflammatory and antioxidant properties. We examined the antioxidant, antidiabetic, anti-obesity, antithyroid, and anticancer activities of methanol, ethanol, and acetone extracts of barnûf leaves. The methanol extract exhibited the highest total phenolic (241.50 ± 3.71 mg GAE/g extract) and flavonoid (256.18 ± 3.19 mg QE/g extract) contents. All three extracts proved to possess good antioxidant, antimicrobial, antidiabetic, anti-obesity, antithyroid, and anticancer activities. Ellagic acid was the most abundant phenolic acid in the methanolic (30.33%) and ethanolic (24.71%) extracts. The antioxidant experiments revealed that the methanolic extract had potent DPPH• (IC50 = 18.21 μg/mL) and ABTS•+ (IC50 = 17.6 μg/mL) scavenging properties. The acetone extract demonstrated the highest antimicrobial activity against gramnegative bacteria. Regarding α-amylase and α-glucosidase inhibition, the methanolic extract showed the most potent activity with IC50 values of 104.28 ± 1.97 and 133.76 ± 2.09 μg/mL, respectively. The methanolic extract also proved to be the strongest inhibitor of lipase and thyroid peroxidase, with IC50 values of 127.35 and 211.2 μg/mL, respectively. In addition, the methanolic extract showed the strongest anticancer activity against MCF7-1 and H1299-1 lines with IC50 values of 29.3 and 18.4 μg/mL, respectively. The findings suggest that barnûf leaf extracts could be used in functional foods and pharmaceuticals.

 Pluchea dioscoridis L. medicinal properties extraction DPPH ABTS herbal medicine Pluchea dioscoridis L. medicinal properties extraction DPPH ABTS herbal medicine

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