Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 90245 10.21603/2308-4057-2025-2-642 Research Article Research Article Research Article Extraction methods: Effects on the contents of bioactive compounds and anti-oxidant activity of Coriolosis aspera mycelia Extraction methods: Effects on the contents of bioactive compounds and anti-oxidant activity of Coriolosis aspera mycelia https://orcid.org/0009-0007-2170-4997 Thu Le Minh Thu Le Minh https://orcid.org/0000-0002-1644-236X Thuan Nguyen Ngoc Thuan Nguyen Ngoc https://orcid.org/0000-0002-8146-1102 Nguyen Luu Thao Nguyen Luu Thao https://orcid.org/0000-0002-3170-0785 Mai Dam Sao Mai Dam Sao https://orcid.org/0000-0002-0081-0930 Phuong Do Viet Phuong Do Viet dovietphuong@iuh.edu.vn Industrial University of Ho Chi Minh City Ho Chi Minh Вьетнам Industrial University of Ho Chi Minh City Ho Chi Minh Vietnam Industrial University of Ho Chi Minh City Ho Chi Minh Вьетнам Industrial University of Ho Chi Minh City Ho Chi Minh Vietnam Industrial University of Ho Chi Minh City Ho Chi Minh Вьетнам Industrial University of Ho Chi Minh City Ho Chi Minh Vietnam Industrial University of Ho Chi Minh City Ho Chi Minh Вьетнам Industrial University of Ho Chi Minh City Ho Chi Minh Vietnam Industrial University of Ho Chi Minh City Ho Chi Minh Вьетнам Industrial University of Ho Chi Minh City Ho Chi Minh Vietnam 01 01 2025 01 01 2025 13 2 355 365 24 11 2023 06 02 2024 https://jfrm.ru/en/issues/22898/22981/

Coriolosis aspera has been known as a medicinal mushroom commonly used in Vietnam, China, and certain regions in South Asia. It has many health-beneficial effects, namely anti-inflammatory, anti-cancerous, and anti-antioxidant. Despite these advantages, the rigid and durable cell walls of C. aspera pose challenges during chemical or mechanical extraction processes. We aimed to identify the optimal method for extracting bioactive compounds from C. aspera among hot-water extraction, ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted alkali extraction, and ultrasound-assisted liquid nitrogen extraction. Among these methods, a combination of liquid nitrogen treatment (with a material-to-nitrogen ratio of 1:6) and ultrasoundassisted extraction (15 min) proved to be the most effective. This method yielded the highest concentrations of polyphenols (4.69 ± 0.02 mg GAE/g dry weight), flavonoids (0.88 ± 0.01 mg QE/g dry weight), and triterpenoids (1.28 ± 0.01 mg OAE/g dry weight). Additionally, it exhibited a notable antioxidant activity of 3.48 ± 0.01 μg acid ascorbic/g Dry weight. The scanning electron microscope images indicated that ultrasound-assisted liquid nitrogen extraction was the only method able to effectively disrupt the cell walls of C. aspera. Our study contributes to the potential application of C. aspera in developing functional foods. It emphasizes the importance of effective extraction techniques in discovering medicinal properties of the mushroom.

Coriolosis aspera has been known as a medicinal mushroom commonly used in Vietnam, China, and certain regions in South Asia. It has many health-beneficial effects, namely anti-inflammatory, anti-cancerous, and anti-antioxidant. Despite these advantages, the rigid and durable cell walls of C. aspera pose challenges during chemical or mechanical extraction processes. We aimed to identify the optimal method for extracting bioactive compounds from C. aspera among hot-water extraction, ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted alkali extraction, and ultrasound-assisted liquid nitrogen extraction. Among these methods, a combination of liquid nitrogen treatment (with a material-to-nitrogen ratio of 1:6) and ultrasoundassisted extraction (15 min) proved to be the most effective. This method yielded the highest concentrations of polyphenols (4.69 ± 0.02 mg GAE/g dry weight), flavonoids (0.88 ± 0.01 mg QE/g dry weight), and triterpenoids (1.28 ± 0.01 mg OAE/g dry weight). Additionally, it exhibited a notable antioxidant activity of 3.48 ± 0.01 μg acid ascorbic/g Dry weight. The scanning electron microscope images indicated that ultrasound-assisted liquid nitrogen extraction was the only method able to effectively disrupt the cell walls of C. aspera. Our study contributes to the potential application of C. aspera in developing functional foods. It emphasizes the importance of effective extraction techniques in discovering medicinal properties of the mushroom.

 Anti-oxidant activity Coriolopsis aspera flavonoids mycelia triterpenoids ultrasound-assisted extraction Anti-oxidant activity Coriolopsis aspera flavonoids mycelia triterpenoids ultrasound-assisted extraction

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