Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 89510 10.21603/2308-4057-2025-2-649 Research Article Research Article Research Article The phytochemical composition of Kuzbass medicinal plants The phytochemical composition of Kuzbass medicinal plants https://orcid.org/0000-0002-9061-1256 Velichkovich Natalia S. Velichkovich Natalia S. velichkovich@yandex.ru https://orcid.org/0000-0002-6158-9854 Dunchenko Nina I. Dunchenko Nina I. https://orcid.org/0000-0001-7774-8859 Stepanova Anna A. Stepanova Anna A. https://orcid.org/0000-0002-2960-0216 Kozlova Oksana V. Kozlova Oksana V. https://orcid.org/0000-0001-9711-2145 Faskhutdinova Elizaveta R. Faskhutdinova Elizaveta R. faskhutdinovae.98@mail.ru https://orcid.org/0000-0002-1779-4332 Yustratov Vladimir P. Yustratov Vladimir P. https://orcid.org/0000-0001-9293-4377 Luzyanin Sergey L. Luzyanin Sergey L. Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Russian State Agrarian University – Moscow Timiryazev Agricultural Academy Moscow Россия Russian State Agrarian University – Moscow Timiryazev Agricultural Academy Moscow Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation 01 01 2025 01 01 2025 13 2 219 232 27 04 2024 06 08 2024 https://jfrm.ru/en/issues/22898/22908/

Flavonoids are plant polyphenols that exhibit biological activity with antibacterial, antiviral, antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic effects. The medicinal plants of Kuzbass have high contents of flavonoids and other polyphenolic compounds. Therefore, they can be used in medicinal preparations to prevent or treat serious diseases. We studied the following plants collected in Kuzbass: common thyme (Thymus vulgaris Linn., leaves and stems), woolly burdock (Arctium tomentosum Mill., roots), alfalfa (Medicago sativa L., leaves and stems), common lungwort (Pulmonaria officinalis L., leaves and stems), common yarrow (Achillea millefolium L., leaves and stems), red clover (Trifolium pratense L., leaves and stems), common ginseng (Panax ginseng, roots), sweetvetch (Hedysarum neglectum Ledeb., roots), and cow parsnip (Heracleum sibiricum L., inflorescences, leaves, and stems). To extract flavonoids, we used ethanol at concentrations of 40, 55, 60, 70, and 75%. Spectrophotometry was used to determine total flavonoids, while high-performance liquid chromatography was employed to study the qualitative and quantitative composition of the extracts. The highest yield of flavonoids was found in H. sibiricum leaves (at all concentrations except 70%), followed by the 55% and 70% ethanol extracts of T. vulgaris leaves and stems, as well as the 75% ethanol extract of A. millefolium leaves and stems. Thus, these plants have the greatest potential in being used in medicines. High-performance liquid chromatography showed the highest contents of polyphenolic compounds in the samples of P. officinalis, A. millefolium, T. vulgaris, and T. pratense. Our results can be used in further research to produce new medicinal preparations based on the medicinal plants of Kuzbass.

Flavonoids are plant polyphenols that exhibit biological activity with antibacterial, antiviral, antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic effects. The medicinal plants of Kuzbass have high contents of flavonoids and other polyphenolic compounds. Therefore, they can be used in medicinal preparations to prevent or treat serious diseases. We studied the following plants collected in Kuzbass: common thyme (Thymus vulgaris Linn., leaves and stems), woolly burdock (Arctium tomentosum Mill., roots), alfalfa (Medicago sativa L., leaves and stems), common lungwort (Pulmonaria officinalis L., leaves and stems), common yarrow (Achillea millefolium L., leaves and stems), red clover (Trifolium pratense L., leaves and stems), common ginseng (Panax ginseng, roots), sweetvetch (Hedysarum neglectum Ledeb., roots), and cow parsnip (Heracleum sibiricum L., inflorescences, leaves, and stems). To extract flavonoids, we used ethanol at concentrations of 40, 55, 60, 70, and 75%. Spectrophotometry was used to determine total flavonoids, while high-performance liquid chromatography was employed to study the qualitative and quantitative composition of the extracts. The highest yield of flavonoids was found in H. sibiricum leaves (at all concentrations except 70%), followed by the 55% and 70% ethanol extracts of T. vulgaris leaves and stems, as well as the 75% ethanol extract of A. millefolium leaves and stems. Thus, these plants have the greatest potential in being used in medicines. High-performance liquid chromatography showed the highest contents of polyphenolic compounds in the samples of P. officinalis, A. millefolium, T. vulgaris, and T. pratense. Our results can be used in further research to produce new medicinal preparations based on the medicinal plants of Kuzbass.

 Flavonoids medicinal plants spectrophotometry chromatography HPLC extraction plant extracts Flavonoids medicinal plants spectrophotometry chromatography HPLC extraction plant extracts Our study was financially supported by the Russian Science Foundation (RSF) (grant No. 23-16-00113). Our study was financially supported by the Russian Science Foundation (RSF) (grant No. 23-16-00113).

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