Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 64517 10.21603/2308-4057-2023-2-582 Research Article Research Article Research Article Microbiological safety criteria for products from unconventional raw materials: raw bear fat Microbiological safety criteria for products from unconventional raw materials: raw bear fat https://orcid.org/0000-0001-6842-4537 Vechtomova Elena A. Vechtomova Elena A. vechtomowa.lena@yandex.ru https://orcid.org/0000-0002-9650-8727 Dolgolyuk Irina V. Dolgolyuk Irina V. https://orcid.org/0000-0002-9410-3662 Orlova Maria M. Orlova Maria M. https://orcid.org/0000-0003-4645-828X Zaushintsena Alexandra V. Zaushintsena Alexandra V. Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation KAO Azot Russia Kemerovo Россия KAO Azot Russia Kemerovo Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation 14 06 2023 14 06 2023 11 2 347 354 13 02 2023 04 04 2023 https://jfrm.ru/en/issues/21476/21650/

Fat of game animals is a prospective raw material for bioactive additives. Before such a product enters the market, food science has to make sure it is safe for consumption. This research featured subcutaneous adipose tissue of brown bears tested with standard methods for microbiological safety indicators. The microbial properties were studied on liquid and solid nutrient media. Staphylococcus was profiled using a VITEK 2 Compact biochemical automatic analyzer and Gram-positive cards (Bio-Mérieux, France). The analysis followed the Technical Regulations of the Customs Union TR TS 021/2011 On Food Safety (December 09, 2011). The microbial count for mesophilic aerobic and facultative anaerobic microorganisms was 1.5×103 CFU/g. The fat samples revealed no molds, yeasts, or Escherichia coli bacteria. Liquid and solid nutrient media made it possible to describe the qualitative profile and cultural properties of the bear fat microflora against pork fat, which served as control. The automatic system identified Gram-positive, coagulase-negative, and oxidase-positive Staphylococcus lentus and Staphylococcus sciuri. In line with the modern classification, they belong to the new genus of Mammaliicoccus, namely Mammaliicoccus sciuri. Subcutaneous adipose tissue of brown bears needs to undergo a microbiological safety test before consumption. Bear fat requires additional research in order to become a safe raw material for food products and bioactive additives.

Fat of game animals is a prospective raw material for bioactive additives. Before such a product enters the market, food science has to make sure it is safe for consumption. This research featured subcutaneous adipose tissue of brown bears tested with standard methods for microbiological safety indicators. The microbial properties were studied on liquid and solid nutrient media. Staphylococcus was profiled using a VITEK 2 Compact biochemical automatic analyzer and Gram-positive cards (Bio-Mérieux, France). The analysis followed the Technical Regulations of the Customs Union TR TS 021/2011 On Food Safety (December 09, 2011). The microbial count for mesophilic aerobic and facultative anaerobic microorganisms was 1.5×103 CFU/g. The fat samples revealed no molds, yeasts, or Escherichia coli bacteria. Liquid and solid nutrient media made it possible to describe the qualitative profile and cultural properties of the bear fat microflora against pork fat, which served as control. The automatic system identified Gram-positive, coagulase-negative, and oxidase-positive Staphylococcus lentus and Staphylococcus sciuri. In line with the modern classification, they belong to the new genus of Mammaliicoccus, namely Mammaliicoccus sciuri. Subcutaneous adipose tissue of brown bears needs to undergo a microbiological safety test before consumption. Bear fat requires additional research in order to become a safe raw material for food products and bioactive additives.

 Brown bear fat subcutaneous adipose tissue microbiological indicators Mammaliicoccus lentus Mammaliicoccus sciuri Brown bear fat subcutaneous adipose tissue microbiological indicators Mammaliicoccus lentus Mammaliicoccus sciuri The research was supported by the Kemerovo State University (KemSU). The research was supported by the Kemerovo State University (KemSU).

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