Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 110932 10.21603/2074-9414-2025-4-2604 UNUDAG ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Effect of Salinity on Secondary Metabolites and Antioxidant Activity in Callus Extracts of Hyssopus officinalis L. Влияние засоления на состав вторичных метаболитов и антиоксидантную активность экстрактов каллусных культур Hyssopus officinalis L. https://orcid.org/0000-0001-7008-3823 Попова Елена Александровна Popova Elena A. elena_popova97@mail.ru Балтийский федеральный университет имени Иммануила Канта Калининград Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation 25 12 2025 25 12 2025 55 4 767 777 18 03 2025 05 08 2025 https://fptt.ru/en/issues/24078/24092/

Рост интереса к поиску новых источников биологически активных веществ делает каллусные культуры лекарственных растений перспективными в области биотехнологий. Особенно востребованными являются каллусные культуры иссопа лекарственного (Hyssopus officinalis L.) благодаря его уникальному химическому составу и свойствам. Важной задачей остается изучение факторов, помогающих повысить синтез необходимых соединений в каллусных культурах. Цель исследования – оценка влияния засоления на рост, содержание фенольных веществ и антиоксидантный потенциал каллусных культур H. officinalis. В качестве объектов исследования выступали каллусные культуры иссопа лекарственного (H. officinalis), культивируемые на трех вариантах среды Мурасиге–Скуга. Содержание фенольных соединений и гидроксикоричных кислот определяли по стандартным методикам. Антиоксидантную активность определяли спектрофотометрическим методом путем анализа активности поглощения радикалов, восстанавливающей способности и хелатной активности. Установлено подавление роста каллусных культур H. officinalis при добавлении высоких концентраций (200–500 мМ) хлорида натрия. Кроме того, эксперимент показал влияние солености питательной среды на количество фенольных соединений в каллусной культуре на среде МС-2. Так, при увеличении солености питательной среды выявлено понижение количества фенольных соединений. При добавлении в питательные среды МС-5 и МС-6 50 и 100 мМ хлорида натрия получен результат, свидетельствующий о наибольшем увеличении фенольных соединений в каллусной культуре. При анализе общего содержания гидроксикоричных кислот, полученных на основании эксперимента с тремя каллусными культурами, зафиксировано достоверное уменьшение их количества при разных концентрациях хлорида натрия. Присутствие в питательной среде различных концентраций соли приводило к понижению антиоксидантной активности экстрактов каллусной культуры по сравнению с контролем. Полученные в ходе исследования результаты показали, что применение солевого стресса для усиления накопления фенольных соединений, гидроксикоричных кислот и повышения антиоксидантной активности экстрактов каллусных культур H. officinalis неэффективно.

Callus cultures of medicinal plants have good prospects for biotechnology as sources of bioactive compounds. Hyssop callus cultures (Hyssopus officinalis L.) possess unique chemical composition and properties. To study the factors that enhance the synthesis of bioactive compounds, the author assessed the effect of salinity on the growth, phenolic content, and antioxidant potential in H. officinalis callus cultures. While the content of phenolic compounds and hydroxycinnamic acids was determined using standard methods, the radical absorption activity, reducing capacity, and chelating activity made it possible to measure the antioxidant activity of the callus cultures. High concentrations (200–500 mM) of NaCl suppressed the callus culture growth. The growth agents included three variants: MS-2 (2 mg/L kinetin, 3 mg/L 1-naphthaleneacetic acid), MS-5 (0.8 mg/L 6-benzylaminopurine, 1.5 mg/L 3-indoleacetic acid), and MS-6 (0.2 mg/L 6-benzylaminopurine and 1 mg/L 2,4-dichlorophenoxyacetic acid). The nutrient medium salinity affected the yield of phenolic compounds in the MS-2 callus culture. As the nutrient medium salinity increased, the amount of phenolic compounds went down. Adding 50 and 100 mM NaCl to MS-5 and MS-6 nutrient media boosted the content of phenolic compounds in the callus culture. The total content of hydroxycinnamic acids pointed at a significant decrease in their amount at different NaCl concentrations. Various salt concentrations in the nutrient medium inhibited the antioxidant activity of the callus culture extracts. In this research, salt stress failed to increase the accumulation of phenolic compounds and hydroxycinnamic acids in H. officinalis. callus culture extracts. It also proved ineffective as antioxidant activity catalyst.

 Лекарственное растение каллус засоление фенольные соединения гидроксикоричные кислоты антиоксидантная активность Medicinal herb callus salinity phenolic substances hydroxycinnamic acids antioxidant activity

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