Food Processing: Techniques and Technology Food Processing: Techniques and Technology Техника и технология пищевых производств 2074-9414 2313-1748 105010 10.21603/2074-9414-2025-3-2590 ОРИГИНАЛЬНАЯ СТАТЬЯ ORIGINAL ARTICLE ОРИГИНАЛЬНАЯ СТАТЬЯ Production of Galacto-Oligosaccharides Using Kluyveromyces Yeast Особенности получения галактоолигосахаридов с использованием дрожжей Kluyveromyces https://orcid.org/0000-0001-9803-8709 Рябцева Светлана Андреевна Ryabtseva Svetlana A. ryabtseva07@mail.ru https://orcid.org/0000-0002-0119-9061 Шпак Мария Александровна Shpack Maria A. https://orcid.org/0000-0002-8200-3007 Сазанова Серафима Николаевна Sazanova Serafima N. Северо-Кавказский федеральный университет Ставрополь Россия North-Caucasus Federal University Stavropol Russian Federation Северо-Кавказский федеральный университет Ставрополь Россия North-Caucasus Federal University Stavropol Russian Federation Северо-Кавказский федеральный университет Ставрополь Россия North-Caucasus Federal University Stavropol Russian Federation 05 08 2025 05 08 2025 55 3 581 606 18 06 2025 21 07 2025 https://fptt.ru/en/issues/23788/23830/

Галактоолигосахариды обладают пребиотической активностью, их получают из лактозы с использованием бета-галактозидаз бактериального или грибного происхождения. Цель обзора – выявление и анализ факторов, влияющих на биосинтез и очистку галактоолигосахаридов, с использованием бета-галактозидаз дрожжей рода Kluyveromyces, а также перспективных направлений исследований в этой области. Объектами исследования стали научные публикации по вопросам получения галактоолигосахаридов с использованием бета-галактозидаз дрожжей. Для поиска информации были использованы международные базы данных Scopus, Web of Science, PubMed, eLIBRARY.RU Для анализа было отобрано 87 источников, в основном статьи в рецензируемых журналах, опубликованные преимущественно за последние 10 лет. Выбор фермента и формы его применения, оптимизация условий биосинтеза и очистки являются наиболее важными для регулирования выхода, состава и свойств анализируемых веществ. Бета-галактозидазы из Kluyveromyces могут одновременно катализировать реакции гидролиза и трансгалактозилирования. Условия протекания процессов биосинтеза галактоолигосахаридов с этими ферментами широко варьируют в разных публикациях, как и их выход, но чаще всего этот показатель не превышает 30–40 %, при этом общая конверсия лактозы достигает 80–90 %. Применение бета-галактозидаз Kluyveromyces в виде цельноклеточных ферментов, в иммобилизованной форме или совместно с другими ферментами позволяет улучшить экономические показатели процессов биосинтеза и / или выход и / или структуру рассматриваемых веществ. Прямой их синтез в молоке или сыворотке дает возможность получать новые функциональные молочные продукты и добавки. Селективная биоконверсия с дрожжами Kluyveromyces позволяет повысить чистоту галактоолигосахаридов до 90 % экономически выгодным и экологически безопасным путем. К перспективным направлениям можно отнести использование побочного молочного сырья для получения продуктов с галактоолигосахаридами, комбинированное применение ферментов разных продуцентов, а также комплексное применение бета-галактозидаз Kluyveromyces для их биосинтеза и очистки.

Galacto-oligosaccharides are known for their prebiotic activity. They are obtained from lactose using bacterial or fungal betagalactosidases. This article describes the factors that affect the biosynthesis and purification of galacto-oligosaccharides using Kluyveromyces yeasts, as well as summarizes some prospective research areas in this sphere. The research covered ten years of scientific publications on the production of galacto-oligosaccharides with yeast beta-galactosidases. The review pool included 87 articles published in peer-reviewed journals and registered in Scopus, Web of Science, PubMed, and eLIBRARY.RU. The yield, composition, and properties of galacto-oligosaccharides depend on the enzyme, its application, biosynthesis optimization, and purification conditions. Beta-galactosidases from Kluyveromyces can simultaneously catalyze hydrolysis and transgalactosylation reactions. The biosynthesis conditions vary a lot across the review pool, as does the yield of galacto-oligosaccharides, which usually remains below 30–40% while the total lactose conversion reaches 80–90%. Kluyveromyces beta-galactosidases can be used as whole-cell enzymes in immobilized form or together with other enzymes. They improve the economic indicators of biosynthesis, and / or the yield and / or the structure of galacto-oligosaccharides. If synthesized directly in milk or whey, galacto-oligosaccharides may yield new functional dairy products and additives. The method of selective bioconversion with Kluyveromyces yeast brings the purity of galacto-oligosaccharides up to 90% in an economical and sustainable way. Eventually, galacto-oligosaccharides can be obtained from dairy by-products. Other promising areas include the enzymic mixes of different producers, as well as a comprehensive use of Kluyveromyces beta-galactosidases for galacto-oligosaccharide biosynthesis and purification.

 Лактоза бета-галактозидазы Kluyveromyces трансгалактозилирование гидролиз молочная сыворотка очистка Lactose beta-galactosidases Kluyveromyces transgalactosylation hydrolysis whey purification Исследование выполнено за счет гранта Российского научного фонда № 25-26-20093, https://rscf.ru/project/25-26-20093/ The work was supported by the grant of the Russian Science Foundation No. 25-26-20093, https://rscf.ru/project/25-26-20093/

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